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Species: Homo sapiens
Genetic Insert: Kif5b(560)-mCherry-eDHFR
Vector Backbone Description:
References:
Comments:
Proper citation: RRID:Addgene_67760 Copy
Species: Homo sapiens
Genetic Insert: PIK3R1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Open state: NO stop codon immediately follows insert
Proper citation: RRID:Addgene_81748 Copy
Species: Homo sapiens
Genetic Insert: PIK3R1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23696 Copy
Species: Homo sapiens
Genetic Insert: P85(T576del)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_40228 Copy
Species: Homo sapiens
Genetic Insert: P85(N564K)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_40226 Copy
Species: Homo sapiens
Genetic Insert: P85(K550_K562dup)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_40224 Copy
Species: Homo sapiens
Genetic Insert: PIK3R1-Ni
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17744 Copy
Species: Homo sapiens
Genetic Insert: P85(KS459delN)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_40222 Copy
Species: Homo sapiens
Genetic Insert: P85(E439del)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_40221 Copy
Species: Homo sapiens
Genetic Insert: p85alpha 3'UTR
Vector Backbone Description: Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:pGL3-UC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: miR-29 seed mutant (TGCT->GTAG)
Proper citation: RRID:Addgene_51393 Copy
Species: Homo sapiens
Genetic Insert: iSH2-pMagFast2(3x)-iRFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5354; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67298 Copy
Species: Homo sapiens
Genetic Insert: p85 alpha (delta BH)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: see author's map for more information.
Proper citation: RRID:Addgene_13431 Copy
Species: Homo sapiens
Genetic Insert: p85a(N)
Vector Backbone Description: Backbone Marker:GE Healthcare Biosciences; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The molecular weight of the GST fusion protein is approximately 38.5 kDa
Proper citation: RRID:Addgene_46464 Copy
Species: Homo sapiens
Genetic Insert: p85a(C)
Vector Backbone Description: Backbone Marker:GE Healthcare Biosciences; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The molecular weight of the GST fusion protein is approximately 38.4 kDa
Proper citation: RRID:Addgene_46463 Copy
Species: Homo sapiens
Genetic Insert: eDrTrkB
Vector Backbone Description: Vector Backbone:AAV2/9-CW3SL; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_184002 Copy
Species: Homo sapiens
Genetic Insert: ensconsin
Vector Backbone Description: Backbone Size:5403; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_190736 Copy
Species: Homo sapiens
Genetic Insert: MAPTau
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdEos-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: . Excitation = 505 / 569; Emission = 516 / 581
Proper citation: RRID:Addgene_57636 Copy
Species: Homo sapiens
Genetic Insert: MAPTau
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdEos-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: . Excitation = 505 / 569; Emission = 516 / 581
Proper citation: RRID:Addgene_57637 Copy
Species: Homo sapiens
Genetic Insert: MAPTau
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mKO2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: . Excitation = 551; Emission = 565
Proper citation: RRID:Addgene_57883 Copy
Species: Homo sapiens
Genetic Insert: mCherry-Rheb(C181S)
Vector Backbone Description: Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192437 Copy
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