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Species: Yarrowia lipolytica
Genetic Insert: UAS1B16-TEF(136) promoter
Vector Backbone Description:
References:
Comments:
Proper citation: RRID:Addgene_44379 Copy
Species: Other
Genetic Insert: 3CL Protease
Vector Backbone Description:
References:
Comments: bioRxiv. 2020 Aug 29. doi: 10.1101/2020.08.29.272864 This plasmid is stored and distributed in NEB Stable. It can also be used to transform NEB 10Beta.
Proper citation: RRID:Addgene_160282 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description:
References:
Comments: R. norvegicus (rat) Alpha-1 Connexin 43. Excitation = 450; Emission = 495
Proper citation: RRID:Addgene_56515 Copy
Species: Other
Genetic Insert: SARS-CoV-2 ORF3a protein
Vector Backbone Description:
References:
Comments: NCBI Reference Sequence: YP_009724391.1 NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149230 Copy
Species: Arabidopsis thaliana
Genetic Insert: pTMO5:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp6:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description:
References:
Comments:
Proper citation: RRID:Addgene_116004 Copy
Species: Homo sapiens
Genetic Insert: Kif5b(560)-mCherry-eDHFR
Vector Backbone Description:
References:
Comments:
Proper citation: RRID:Addgene_67760 Copy
Species: Other
Genetic Insert: SARS-CoV-2 ORF8 protein
Vector Backbone Description:
References:
Comments: NCBI Reference Sequence: YP_009724396.1
Proper citation: RRID:Addgene_145689 Copy
Species: Other
Genetic Insert: SARS-CoV-2 nsp4
Vector Backbone Description:
References:
Comments: NCBI Reference Sequence: YP_009725300.1 NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149254 Copy
Species: Homo sapiens
Genetic Insert: PIK3R1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Open state: NO stop codon immediately follows insert
Proper citation: RRID:Addgene_81748 Copy
Species: Homo sapiens
Genetic Insert: PIK3R1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23696 Copy
Species: Homo sapiens
Genetic Insert: P85(T576del)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_40228 Copy
Species: Homo sapiens
Genetic Insert: P85(N564K)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_40226 Copy
Species: Homo sapiens
Genetic Insert: P85(K550_K562dup)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_40224 Copy
Species: Mus musculus
Genetic Insert: Axin
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21287 Copy
Species: Homo sapiens
Genetic Insert: PIK3R1-Ni
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17744 Copy
Species: Homo sapiens
Genetic Insert: P85(KS459delN)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_40222 Copy
Species: Homo sapiens
Genetic Insert: P85(E439del)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6949; Vector Backbone:pLenti4-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_40221 Copy
Species: Homo sapiens
Genetic Insert: p85alpha 3'UTR
Vector Backbone Description: Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:pGL3-UC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: miR-29 seed mutant (TGCT->GTAG)
Proper citation: RRID:Addgene_51393 Copy
Species: Homo sapiens
Genetic Insert: iSH2-pMagFast2(3x)-iRFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5354; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67298 Copy
Species: Synthetic
Genetic Insert: FRT-mCherry-attP-BleoR-F3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183755 Copy
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