Searching the Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Niches are often found in specific positions in tissues relative to the stem cells they support. Consistency of niche position suggests that placement is important for niche function. However, the complexity of most niches has precluded a thorough understanding of how their proper placement is established. To address this, we investigated the formation of a genetically tractable niche, the Drosophila Posterior Signaling Center (PSC), the assembly of which had not been previously explored. This niche controls hematopoietic progenitors of the lymph gland (LG). PSC cells were previously shown to be specified laterally in the embryo, but ultimately reside dorsally, at the LG posterior. Here, using live-imaging, we show that PSC cells migrate as a tight collective and associate with multiple tissues during their trajectory to the LG posterior. We find that Slit emanating from two extrinsic sources, visceral mesoderm and cardioblasts, is required for the PSC to remain a collective, and for its attachment to cardioblasts during migration. Without proper Slit-Robo signaling, PSC cells disperse, form aberrant contacts, and ultimately fail to reach their stereotypical position near progenitors. Our work characterizes a novel example of niche formation and identifies an extrinsic signaling relay that controls precise niche positioning.
Pubmed ID: 39750120
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Software tool for automated microscope acquisition, device control, and image analysis. Used for integrating dissimilar fluorescent microscope hardware and peripherals into a single custom workstation, while providing all the tools needed to perform analysis of acquired images. Offers user friendly application modules for analysis such as cell signaling, cell counting, and protein expression.
View all literature mentionsDatabase of Drosophila genetic and genomic information with information about stock collections and fly genetic tools. Gene Ontology (GO) terms are used to describe three attributes of wild-type gene products: their molecular function, the biological processes in which they play a role, and their subcellular location. Additionally, FlyBase accepts data submissions. FlyBase can be searched for genes, alleles, aberrations and other genetic objects, phenotypes, sequences, stocks, images and movies, controlled terms, and Drosophila researchers using the tools available from the "Tools" drop-down menu in the Navigation bar.
View all literature mentionsA commercial antibody vendor, specializing in secondary antibodies.
View all literature mentionsA commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.
View all literature mentionsAn Antibody supplier and subset of ThermoFisher Scientific which provides fluorescence reagents for various experiments and methods.
View all literature mentionsAn antibody supplier which banks and distributes hybridomas and monoclonal antibodies for use in research. The bank includes antibodies against targets such as GFP, transcription factors, stem cells, and human.
View all literature mentionsThis polyclonal targets beta Galactosidase
View all literature mentionsThis monoclonal targets slit protein, C-terminal
View all literature mentionsThis monoclonal targets Mouse Drosophila Antennapedia protein
View all literature mentionsThis monoclonal targets Fasciclin III (Drosophila)
View all literature mentionsThis polyclonal targets Mef2
View all literature mentionsThis unknown targets Donkey Serum
View all literature mentionsThis polyclonal targets GFP-1010
View all literature mentionsThis polyclonal targets RFP
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(2R)BSC699, P+PBac{w[+mC]=XP3.RB5}BSC699/SM6a from BDSC.
View all literature mentionsCore is division of Genetics Core Facilities, University service center to provide consultation, training, and services in areas of cell culture and hybridomas. Stockroom serves University of Pennsylvania investigators and affiliate institutions by coordinating relations with various suppliers of molecular biological research materials. This involves not only bulk purchasing of these products, but negotiation of discounts and convenient delivery arrangements. Special ordering of non regularly stocked products is available from 28 bioreagent vendors with discounted pricing and overnight delivery.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{bap-GAL4.3}1.1 from BDSC.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=UAS-hid.Z}2/CyO from BDSC.
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23]; Mi{PT-GFSTF.1}robo2[MI04295-GFSTF.1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=10XUAS-IVS-myr::GFP}su(Hw)attP1/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=10XUAS-IVS-myr::GFP}su(Hw)attP1/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+m*]=tinC-Gal4.Delta4}12a from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF01229}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; M{RFP[3xP3.PB] w[+mC]=10xUAS-HA-robo1.DeltaC}ZH-86Fb from BDSC.
View all literature mentionsCore provides personalized assistance on all aspects of imaging, from tips on sample preparation to training on one of our microscopes to assistance with image data analysis. Facility houses confocal microscopes, Bruker Vutara 352 super resolution system, Zeiss Z.1 Lightsheet system, three widefield light microscopes, and several computers dedicated to image processing and analysis. Offers scanning electron microscope sample preparation and imaging.
View all literature mentionsDrosophila melanogaster with name ru[1] Diap1[th-1] st[1] kni[ri-1] rn[roe-1] p[p] e[s] bap[208] ca[1]/TM3, P{ry[+t7.2]=ftz-lacZ.ry[+]}TM3, Sb[1] ry[*] from BDSC.
View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=HZ}svp[3] ry[506]/TM3, Sb[1] ry[RK] from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=UAS-hid.Z}2/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-Dcr-2.D}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-Dcr-2.D}10 from BDSC.
View all literature mentionsDrosophila melanogaster with name cn[1] sli[2] bw[1] speck[1]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
View all literature mentions