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In vivo detection of optically-evoked opioid peptide release.

Ream Al-Hasani | Jenny-Marie T Wong | Omar S Mabrouk | Jordan G McCall | Gavin P Schmitz | Kirsten A Porter-Stransky | Brandon J Aragona | Robert T Kennedy | Michael R Bruchas
eLife | 2018

Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.

Pubmed ID: 30175957

Associated grants

  • Agency: NINDS NIH HHS, United States
    Id: P30 NS057105
  • Agency: NIDA NIH HHS, United States
    Id: R00 DA038725
  • Agency: NIDA NIH HHS, United States
    Id: K99 DA038725
  • Agency: NIMH NIH HHS, United States
    Id: F31 MH101956
  • Agency: NIBIB NIH HHS, United States
    Id: R01 EB003320
  • Agency: NIDA NIH HHS, United States
    Id: T32 DA007268
  • Agency: NIDA NIH HHS, United States
    Id: K99/R00 Pathway to Independence DA038725
  • Agency: NIDA NIH HHS, United States
    Id: R01 DA033396

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Promega (tool)

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Software package used for interactive, or batched, statistical analysis in social science, health sciences and marketing. Software platform offers advanced statistical analysis, a library of machine-learning algorithms, text analysis, open-source extensibility, integration with big data and deployment into applications.Versions that were produced by SPSS Inc. before the IBM acquisition (Versions 18 and earlier) would be given origin or publisher of SPSS Inc. in Chicago.

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