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Species: Homo sapiens
Genetic Insert: RelA
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3b parts are generally reserved for c-terminal coding sequences in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123953 Copy
Species: Homo sapiens
Genetic Insert: pCMV
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 2 parts are promoters in the MTK. Promotor sequence from https://doi.org/10.1016/j.ab.2012.06.001 Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123715 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3b parts are used for coding sequences in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123805 Copy
Species: Homo sapiens
Genetic Insert: NFATC2
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3b parts are used for coding sequences in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123804 Copy
Species: Homo sapiens
Genetic Insert: E-Cadherin
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3 parts are generally reserved for coding sequences in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123749 Copy
Species: Homo sapiens
Genetic Insert: alpha tubulin 1b (human
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3b parts are used for coding sequences in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123783 Copy
Species: Homo sapiens
Genetic Insert: H2B
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3a parts are used for N-terminal coding sequences in the MTK system Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123770 Copy
Species: Homo sapiens
Genetic Insert: gRNA-GFPDROPOUT-hu6_20N_cr3
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 234 parts are coupled promoter-coding sequence-terminator such as guide RNA cassettes, GFP dropouts in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123921 Copy
Species: Homo sapiens
Genetic Insert: LA_hROSA26
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 8b parts can be components of the destination backbone such as locus upstream homology arms, resistance cassette, recognition sites, and nuclear marker in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123891 Copy
Species: Homo sapiens
Genetic Insert: LA_hThal5.10-2-hg18
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 8b parts can be components of the destination backbone such as locus upstream homology arms, resistance cassette, recognition sites, and nuclear marker in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123896 Copy
Species: Homo sapiens
Genetic Insert: LA_hAAVS1
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 8b parts can be components of the destination backbone such as locus upstream homology arms, resistance cassette, recognition sites, and nuclear marker in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123898 Copy
Species: Homo sapiens
Genetic Insert: LA_hCLYBL
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 8b parts can be components of the destination backbone such as locus upstream homology arms, resistance cassette, recognition sites, and nuclear marker in the MTK Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123897 Copy
Species: Homo sapiens
Genetic Insert: 2 copies of the p53 DNA binding motif
Vector Backbone Description: Backbone Marker:Self made; Derived from pBluescriptSK; Backbone Size:2343; Vector Backbone:pUPD; Vector Types:Synthetic Biology, Other, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
References:
Comments: Insert can be released with BsaI
Proper citation: RRID:Addgene_118053 Copy
Species: Homo sapiens
Genetic Insert: Elongin BC
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Full length Elongin B was cloned into MCS1 NcoI site of pACYC-Duet1.
Elongin C (a.a. 17-112) was cloned in 2nd MCS with stop codon before any 3’ vector S tag. Therefore, these inserts produce untagged proteins.
Note: ATG from NdeI site precedes internal start Met17 of Elongin C giving start sequence MMYVK...
2C9W: https://www.thesgc.org/structures/2C9W.
Proper citation: RRID:Addgene_110274 Copy
Species: Homo sapiens
Genetic Insert: A3A-W98Y
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Other; Bacterial Resistance:Chloramphenicol
References:
Comments: Along with pBT195, this plasmid can be used to clone pBT361 by SapI or LguI restriction/ligation (Golden Gate). pBT361 is mutagenic in bacteria and care must be taken during cloning, handling and propagation. Benchling map: https://benchling.com/s/seq-viWaBa6R2LjXykdEZnZo
Proper citation: RRID:Addgene_127846 Copy
Species: Homo sapiens
Genetic Insert: Lucia Luciferase
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2343; Vector Backbone:pUPD3; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Insert can be released with BsaI.
Synthetic luciferase from Invivogen, codon-optimized and synthesized de novo.
Proper citation: RRID:Addgene_135287 Copy
Species: Homo sapiens
Genetic Insert: AASDHPPT
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pCOEX-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: http://www.thesgc.org/structures/2BYD/
Proper citation: RRID:Addgene_39192 Copy
Species: Homo sapiens
Genetic Insert: p21 promoter deletion mutant
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5600; Vector Backbone:pBlueScript II KS(+); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_21728 Copy
Species: Homo sapiens
Genetic Insert: p21 promoter deletion mutant
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5600; Vector Backbone:pBlueScript II KS(+); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_21730 Copy
Species: Homo sapiens
Genetic Insert: Cep123
Vector Backbone Description: Backbone Marker:Modified Clontech vector; Backbone Size:5781; Vector Backbone:pLP-mEos2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_72589 Copy
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