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Species: Gallus gallus
Genetic Insert: SIINFEKL
Vector Backbone Description: Backbone Size:8200; Vector Backbone:PresentER; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint.
Proper citation: RRID:Addgene_102945 Copy
Species: Gallus gallus
Genetic Insert: Fusion Glycoprotein type E (P440E)
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCAGGS vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS was unable to fully resolve the sequence of the CAGGS promoter. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_119978 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-851
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site.
Proper citation: RRID:Addgene_46172 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-258
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site.
Proper citation: RRID:Addgene_46173 Copy
Species: Gallus gallus
Genetic Insert: Vcl
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_46171 Copy
Species: Gallus gallus
Genetic Insert: Vcl 884-1066
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site.
Proper citation: RRID:Addgene_46176 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-851 A50I
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: See Addgene plasmid 46172 for wild-type cloning information. The A50I mutation was introduced by QuikChange mutagenesis (Stratagene).
Proper citation: RRID:Addgene_46174 Copy
Species: Gallus gallus
Genetic Insert: paxillin-Electra2
Vector Backbone Description: Backbone Size:3933; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_184926 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-258 A50I
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: See Addgene plasmid 46173 for wild-type cloning information. The A50I mutation was introduced by QuikChange mutagenesis (Stratagene).
Proper citation: RRID:Addgene_46175 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-1066
Vector Backbone Description: Backbone Marker:described in N. Kioka. 1999. JCB 144:59-69; Vector Backbone:p401F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_46284 Copy
Species: Gallus gallus
Genetic Insert: Gallus gallus vinculin
Vector Backbone Description: Vector Backbone:pET15b/GVcl 1-1066 (Addgene #46171); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_162781 Copy
Species: Gallus gallus
Genetic Insert: CCPB1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mEos3.2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: . Excitation = 507 / 572; Emission = 516 / 580
Proper citation: RRID:Addgene_57448 Copy
Species: Gallus gallus
Genetic Insert: NgCam
Vector Backbone Description: Vector Backbone:pBeta Actin-Halo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_162719 Copy
Species: Gallus gallus
Genetic Insert: Paxillin
Vector Backbone Description: Vector Backbone:pmEOS2-Paxillin; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_114192 Copy
Species: Gallus gallus
Genetic Insert: VinculinTS (I997A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5363; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111829 Copy
Species: Gallus gallus
Genetic Insert: VinculinTS (I997A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5360; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111828 Copy
Species: Gallus gallus
Genetic Insert: Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5399; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Partially based on Addgene Plasmids #26019, #49089, and #21769
Proper citation: RRID:Addgene_111764 Copy
Species: Gallus gallus
Genetic Insert: Y-RAN-RFP6
Vector Backbone Description: Backbone Marker:Takara/Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_106424 Copy
Species: Gallus gallus
Genetic Insert: capping protein alpha 1 beta 1 (deletion 28 amino acids )
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:5000; Vector Backbone:pET- 3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Soeno et al 1998; journal of muscle research and cell motility 19, 639-646
Proper citation: RRID:Addgene_13300 Copy
Species: Gallus gallus
Genetic Insert: capping protein alpha 1 (deletion 28 amino acids) beta 1 subunits
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:5000; Vector Backbone:pET- 3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Soeno et al 1998; journal of muscle research and cell motility 19, 639-646
Proper citation: RRID:Addgene_13299 Copy
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