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URL: http://floresta.eead.csic.es/primers4clades
Proper Citation: primers4clades (RRID:SCR_015714)
Description: Web application for the design of PCR primers for cross-species amplification of novel sequences from metagenomic DNA or from uncharacterized organisms belonging to user-specified phylogenetic lineages. It implements an extended CODEHOP strategy and evaluates thermodynamic properties of the oligonucleotide pairs.
Resource Type: software resource, web application
Defining Citation: PMID:19465390
Keywords: pcr primer, metagenomic dna, phylogenetic lineage, primer, clade, bio.tools
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