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Resource Name
PrimerSeq
RRID:SCR_003295 RRID Copied      
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PrimerSeq (RRID:SCR_003295)
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Resource Information

URL: http://primerseq.sourceforge.net/

Proper Citation: PrimerSeq (RRID:SCR_003295)

Description: Software that designs RT-PCR primers that evaluate alternative splicing events by incorporating RNA-Seq data. It is particularly advantageous for designing a large number of primers for validating alternative splicing events found in RNA-Seq data. It incorporates RNA-Seq data in the design process to weight exons by their read counts. Essentially, the RNA-Seq data allows primers to be placed using actually expressed transcripts. This could be for a particular cell line or experimental condition, rather than using annotations that incorporate transcripts that are not expressed for the data. Alternatively, you can design primers that are always on constitutive exons. PrimerSeq does not limit the use of gene annotations and can be used for a wide array of species.

Abbreviations: PrimerSeq

Synonyms: Primer Seek in RNA-Seq

Resource Type: software resource

Defining Citation: PMID:24747190

Keywords: primer, rna-seq, rt-pcr, windows, mac os x, bio.tools

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This resource

is listed by

OMICtools

is listed by

bio.tools

is listed by

Debian

has parent organization

SourceForge

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