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URL: http://primerseq.sourceforge.net/
Proper Citation: PrimerSeq (RRID:SCR_003295)
Description: Software that designs RT-PCR primers that evaluate alternative splicing events by incorporating RNA-Seq data. It is particularly advantageous for designing a large number of primers for validating alternative splicing events found in RNA-Seq data. It incorporates RNA-Seq data in the design process to weight exons by their read counts. Essentially, the RNA-Seq data allows primers to be placed using actually expressed transcripts. This could be for a particular cell line or experimental condition, rather than using annotations that incorporate transcripts that are not expressed for the data. Alternatively, you can design primers that are always on constitutive exons. PrimerSeq does not limit the use of gene annotations and can be used for a wide array of species.
Abbreviations: PrimerSeq
Synonyms: Primer Seek in RNA-Seq
Resource Type: software resource
Defining Citation: PMID:24747190
Keywords: primer, rna-seq, rt-pcr, windows, mac os x, bio.tools
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