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RNA extraction kit from formalin-fixed paraffin-embedded tissue
Nectin4 Gene in Situ hybridization probe and centromeric control regions to determine copy number alterations of NECTIN4 gene relative to control centromeric region.
CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.
Endothelial Cell Medium (ECM), when used with Endothelial Cell Growth Supplement (ECGS, Cat #1052) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal endothelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal endothelial cells in vitro.
The Griess analysis method is the most commonly used and simplest technique for detecting NO concentrations. This method works by summarizing the nitrogen coupling between diazo compounds, which are formed from sulfonamides with NO2 and naphthyl diamine. The nitrate 2/ nitrite 2 test kit includes various dyes, a nitrate reductase, an enzyme cofactor, a buffer solution, and standard solutions of NO2 and NO3.
The MCE reactive oxygen species (ROS) detection kit uses the fluorescent probe DCFH-DA to detect ROS. The principle of detection is that DCFH-DA initially has no fluorescence and can penetrate the cell membrane, where it is hydrolyzed by esterases within the cell to form DCFH, which is impermeable to the cell membrane. ROS in the cell can oxidize the non-fluorescent DCFH to produce fluorescent DCF. The fluorescence signal of DCF is detected using fluorescence microscopy, flow cytometry, or laser confocal microscopy to analyze the level of ROS within the cell.
Abbkine LDH Cytotoxicity Assay Kit provides a simple and easy colorimetric assay for the study of cytotoxicity. This assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm.
This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse sICAM-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sICAM-1 present in the samples binds to the solid-phase antibodies and the detection antibodies.
This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse sVCAM-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate.
Reagent for total RNA extraction from cells or tissues.
The BCA Protein Assay Kit is developed based on the BCA method, one of the two most widely used protein quantification techniques in the world. It enables simple, highly stable, sensitive, and compatible protein concentration measurements.
Cleavage Under Target & Tagmentation (CUT&Tag®) is an emerging technique for studying DNA-protein interactions. The core of CUT&Tag® relies on Tn5 transposase fused to protein A/G. This fusion protein binds to an antibody via protein A/G, thereby tethering Tn5 near the target sites. The tethered Tn5 then performs targeted DNA cleavage and tagmentation, simultaneously introducing sequencing adapters required for next-generation sequencing (NGS). The resulting DNA fragments are extracted and amplified by PCR, generating a sequencing-ready library without additional fragmentation or end-repairing steps
MobaXterm is your ultimate toolbox for remote computing. In a single Windows application, it provides loads of functions that are tailored for programmers, webmasters, IT administrators and pretty much all users who need to handle their remote jobs in a more simple fashion.
Modular multimode microplate reader, with monochromator-based optics and filter-based optics for fluorescence and luminescence detection, plus filter-free UV-Vis absorbance. Synergy H1 offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection. The bandwidth can be set between 9 and 50 nm, in 1 nm increments, to fully optimize reader settings for best sensitivity and specificity compared to fixed bandwidth systems.
LABO is an experiment design tool that researchers can use for creating 2D/3D stimulus presentation tasks on desktop, mobile, and Extended Reality (XR) devices.
PanScan is a computational tool that offers advanced capabilities for identifying novel sequences, SVs, and repetitive regions. With its ability to annotate duplicate genes and visualize complex genomic landscapes, PanScan provides invaluable insights into genetic diversity.
This original version of the Ultra™ RNA Library Prep Kit enables non-directional RNA library preparation in a streamlined workflow. However, we now recommend use of the more recent NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770), which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts.
High ALDH expression has been reported for normal and cancer precursor cells of various lineages. The ALDEFLUOR assay is a widely published non-immunological method for the detection of ALDH-bright (ALDHbr) cells and can be used to detect cancer cells in hematopoietic, mammary, endothelial, mesenchymal, neural, and other tissues.