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Center for research to understand the network of cells that cause benign prostatic hyperplasia , the most common cause of urinary bother in men.
Services include use of high throughput screening technology, compounds in various libraries, siRNA sets of druggable genome for mouse and human and database of results from screens. For compounds screens, data points are in singlets.For functional genomics screens we use duplicates. We typically utilize 384 well plates but are flexible in this regard. We can accomodate any plate type ranging from 96 to 1536 well plates. Specialty plates can be handled on needed base. We have special rates for consumables with many vendors based on volume we use. These reduced rates are passed on directly to our screeners.
Facility is ISO Class 5 cleanroom that specializes in advanced lithographic processing of hard and soft materials. Facility is focused on supporting basic science, applied research, research and development, and prototype production using micro and nanofabrication.
Facility assists researchers in mammalian genome editing and molecular biology, including CRISPR genetic libraries and screens as well as custom viral vector/particle production. Projects are customized to researchers needs, from full service cell line creation to design and cloning.Supported by Canadian Foundation for Innovation.
Provides computational and storage tools needed to efficiently and effectively process data. High performance computing cluster housing compute cores, graphical processing unit cores, and directly attached high speed storage.
Source code for "Accurate and Fast Clade Assignment via Deep Learning and Frequency Chaos Game Representation".
Offers training, assisted and unassisted use of microscopes and equipment, as well as consultations and collaborations. Support includes single particle cryo-EM, Cryo-ET, Micro-ED,simple characterization of samples in vitrified aqueous mixtures (e.g. nanoparticle size, etc.).
Core provides services for generation of transgenic and knockout mice.Provides Nikon Diaphot microscope equipped with Nomarski Optics and Narishige micromanipulators for microinjections, Nikon surgical microscopes for egg isolation and transfer, sutter needle puller and de Fonbrune microforge. Facility maintains mice necessary for egg donors, egg recipients, and vasectomized males.Core offers electroporation of targeting vectors into ES cells. Provides investigators with DNA to identify ES cells carrying desired recombination events and ES cell expansion for microinjection into blastocysts.Services also include CRISPR injections, sperm and embryo cryopreservation, and reanimation with fresh or frozen sperm via IVF or thawing and implanting embryos.
Software package that enables signature classification of tumor samples by generating heatmaps using transcriptomic data.
Open source software package for statistical programming language R to analysis correlation of different data.
Provides light stable isotope ratio analysis services to scientists from UCSC and from around the world.
Data portal hosting access to behavioral data sets generated by International Brain Laboratory aiming to understand brain functions underlying decision making. Datasets are acquired in neuroscience laboratories performing experimental work. In each of these laboratories, mice are first trained in IBL decision making task following standardized training pipeline.
Provides Zeiss LSM880 upright two photon confocal microscope with Airyscan, FAST model and CLARITY lens; LSM880 inverted live cell imaging confocal microscope with Airyscan and FAST model; Agilent Seahorse XFe24 live cell metabolic analyzer.Supported applications include in vivo imaging of live animals, time lapse live cell imaging with high resolution optical sectioning, deep imaging of fixed CLARITY tissues, calcium imaging, photo switching and photo uncaging, Fluorescence Recovery After Photobleaching, Forster Resonance Energy Transfer, laser ablation, and measuring mitochondrial respiration and glycolysis in live cells in real time.Provides advanced imaging analysis software Imaris for data processing.
ETSG serves University of Chicago faculty led research projects. ETSG staff is available to help plan, design, build, test, and install mechanical and electro mechanical apparatus.
Software package in Python for statistical analysis of Ecological Momentary Assessment data.
Software tool as quality assurance workflow for Brain Imaging Data Structure compliant anatomical and functional MRI datasets. Used to extract no-reference image quality metrics from structural and functional magnetic resonance imaging data.
Open source PhysiCell based software package that allows users to include pharmacokinetics and pharmacodynamics in PhysiCell models. Used for module template for drug treatment in PhysiCell.
Open source Python based hardware and software for fiber photometry data acquisition. System includes compact, low cost, data acquisition board built around Micropython microcontroller, and cross platform graphical user interface for controlling acquisition and visualising signals. Can acquire two analog and two digital signals, and control two external LEDs via built in LED drivers. Time division multiplexed illumination allows independent readout of fluorescence evoked by different excitation wavelengths from single photoreceiver signal.
Software tool as diagramming application that enables collaboration in real time.
DARC facility engages directly with faculty members, preparing large scale datasets, assisting with data analysis, and consulting on research design. Provides expertise on machine learning, text processing, and cloud services. DARC team also supports Yen compute environment, linux cluster designed for data intensive workloads in business research.