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Software R package for unbiased cell type recognition of scRNA-seq data. Performs unbiased cell type recognition from single-cell RNA sequencing data, by leveraging reference transcriptomic datasets of pure cell types to infer cell of origin of each single cell independently.
Software R library to automate labor intensive actigraphy data preprocessing and analyses steps while improving transparency, reproducibility, and scalability over software suites traditionally used in actigraphy research practice. Provides streamlined preprocessing while validating circadian rhythm and sleep analysis.
Provides investigators with access to advanced tools for measuring biomolecular interactions and properties through variety of techniques from high throughput screening via automation to single reaction cuvette based methods.Facility complements technologies and techniques available through our Imaging facility and Proteomics and Metabolomics facility.
Sequencing Core offers Illumina next generation sequencing services to University of Pittsburgh research community. Offers expertise in next generation sequencing technologies. Encourages to contact Assistant Director to arrange for consultation.Staff is available to share their experience and expertise to assist with development of projects of all sizes, standard or custom assay design, and other specialized application needs.
Non coding RNA sequence database. Comprehensive ncRNA sequence collection representing all ncRNA types from broad range of organisms.
The GEN-ERA toolbox can be used to infer completely reproducible comparative genomic and metabolic analyses on prokaryotes and small eukaryotes.
Software toolbox to infer completely reproducible comparative genomic and metabolic analyses on prokaryotes and small eukaryotes.
Software package for QC and visualization of ATAC-seq results. Used to examine aligned reads and report basic metrics, including reads mapped in proper pairs, optical or PCR duplicates, reads mapping to autosomal or mitochondrial references, ratio of short to mononucleosomal fragment counts, mapping quality, various kinds of problematic alignments.
Commercial collaborative research platform for hosting and analyzing datasets.Data platform for academic research. Redivis provides organizations with central hub where researchers can easily discover, access, and analyze their data.
Open atlas based on single cell profiling technologies with quantitative neuropathology and deep clinical phenotyping from middle temporal gyrus from neurotypical reference brains and brains from SEA-AD aged cohort that span spectrum of Alzheimer’s disease. Produced via collaboration between Allen Institute for Brain Science, University of Washington Alzheimer Disease Research Center and Kaiser Permanente Washington Health Research Institute.
Web too that implements various methods for evaluating extent of agreement among two or more raters. For two raters, data can be organize either as contingency table (for categorical ratings only) or as two column table of raw categorical or quantitative ratings. For three raters or more, data can be in form of columns of raw scores (for categorical or quantitative ratings), or alternatively in form of distribution of raters by response category for categorical only. Data can be captured in two ways. You can key in ratings directly in data grid or import it from CSV text file or MS Excel. You can highlight portion of grid you want to analyze and click on red action button. Selected data will be described below associated red action button.
Provides services to create, rederive or cryopreserve mouse models.Specific services include:CRISPR/Cas9-mediated gene editing (experimental design service and/or consulting also provided); pronuclear Injection; ES Cell Injection; In Vitro Fertilization; rederivation of mouse lines to specific pathogen free status; embryo and sperm cryopreservation and recovery.
Core provides latest sequencing technology combined with Institute's expansive bioinformatics capabilities. Provides instrumentation including Illumina Miseq, Covaris Model E220, NovaSeq 6000, Agilent 2200 Tapestation, Beckman Coulter Biomek FXP, HiSeq 2500.
Provides instrumentation and technical expertise to local researchers seeking to conduct MS-based protein or small molecule assays.Staff provide assistance with project design, sample preparation, collection and interpretation of mass spectrometric data.Common analyses services include identification of unknown proteins, characterization of protein complexes, mapping of post-translational modifications and relative quantitation of peptides and small molecules from range of sample types, including complex clinical matrices, tissue extracts and cell lysates. Specialized MS instrumentation include Fusion Lumos Tribrid Orbitrap and Thermo Q Exactive Plus Hybrid Quadrupole-Orbitrap. In addition, the laboratory has access to a variety of automated database searching software as well as resources to facilitate discovery-based proteomics projects.
Core has developed library of natural product extracts derived from unique collection of diverse marine and terrestrial actinomycetes, fungi and cyanobacteria. Provides technology and expertise to develop candidates identified through high-throughput screening into unique, bioactive, patentable, small molecules.
Provides high throughput screening of extensive small molecule, natural product and siRNA libraries along with assay development and optimization for basic biology and drug discovery projects.
Software R package for post alignment quality assessment of ATAC-seq data. Package also contains functions to preprocess aligned ATAC-seq data for subsequent peak calling.
Comprehensive resource of neuropeptides, which holds non-redundant neuropeptide entries. Data collected from resources including MEDLINE abstracts, full papers, UniProt,database at www.neuropeptides.nl and Neuropedia. Contains detailed annotations for each entry, including source organisms, tissue specificity, families, names, post-translational modifications, 3D structures and literature references. Amino acid compositions, isoelectric points, molecular weight and other physicochemical properties of peptides are also provided. Search database with keywords such as sequence, name, family, etc.,User friendly web tools like browsing, sequence alignment and mapping are also integrated.Users can submit new entries online. Each new entry is validated before incorporating it.
Software multistage analysis pipeline which progressively discovers cell types or states while mitigating impact of technical artifacts.Used for single cell analysis.