Curator Dashboard

Azure Cielo 3 is 96 well Real Time-PCR Machine available with 3 sample channels. Used for RT-PCR amplification and SYBR Green fluorescence detection. Engineered for variety of qPCR applications, with high sensitivity and wide dynamic range. Fast qPCR detection system for high-quality data with smart protocols, and robust durability.

• Resource
• RRID
• 4 months ago - by Anonymous

Multichannel, multimodal fluorescent imager, with IR, visible light, and UV excitation channels. Able to image and quantify two different targets in the same position more efficiently using near-infrared (NIR). Normalize to fluorescent total protein stain or a housekeeping protein in the green channel without needing to strip and re-probe your Western blots.

• Resource
• RRID
• 4 months ago - by Anonymous

Imaging system capable of three-channel visible fluorescence detection. Enables sensitive multiplex detection of Western blots, fluorescent biomolecules and Cy2/Cy3/Cy5 or similar fluorochromes. Allows you to simultaneously image and quantify up to three different targets.

• Resource
• RRID
• 4 months ago - by Anonymous

Imaging system with the same sensitivity as chemiluminescent detection on film, but without need for darkroom and the expense of film developer.

• Resource
• RRID
• 4 months ago - by Anonymous

Touchscreen-based gel documentation imaging system. It is designed for UV, color imaging, blue-excited DNA dyes, and Coomassie gel imaging.

• Resource
• RRID
• 4 months ago - by Anonymous

Filter-based 96-well plate absorbance microplate reader for ELISA, Bradford, Lowry assays and more.

• Resource
• RRID
• 4 months ago - by Anonymous

Software tools for working with Bisulfite Sequencing data while preserving reads intrinsic dependencies.

• Resource
• RRID
• 4 months ago - submitted by ting qi

Software tool for calculating sequence depth.

• Resource
• RRID
• 4 months ago - submitted by ting qi

On-line viewer for human striatum slide-tags spatial transcriptomics (10X Chromium 5' GEX) data set generated by the Macosko Lab comprising ~1.1 million cells from 19 human striatum donors.

• Resource
• dkNET
• 4 months ago - submitted by Maryann Martone

Structured schema used in single-cell biology to standardize how cell types are annotated, classified, and stored. It is frequently associated with the Allen Institute and Brain Initiative Cell Census Network (BICAN) to map cell taxonomy, linking cell sets to ontologies and providing evidence for annotations

• Resource
• RRID
• 4 months ago - submitted by Edyta Vieth

Loom is an efficient HDF5-based file format for storing large, high-dimensional single-cell genomics datasets, including data, metadata, and clustering results. Loom file format is designed to efficiently hold large omics datasets. Typically, such data takes the form of a large matrix of numbers, along with metadata for the rows and columns. Loom files (.loom) are created in the HDF5 file format, which supports an internal collection of numerical multidimensional datasets. HDF5 is supported by many computer languages, including Python, R, MATLAB, Mathematica, C, C++, Java, and Ruby.

• Resource
• RRID
• 4 months ago - submitted by Edyta Vieth

Software package that allows simultaneous analysis of associations between genomic region sets, enabling clustering of data and creation of graphs. Incorporates strategy to improve p-value calculations and normalize z-scores coming from multiple analysis to allow for their direct comparison. Builds upon regioneR by adding new plotting functions for obtaining publication-ready graphs.

• Resource
• RRID
• 4 months ago - submitted by Mitchell Cheung

Software R package to work with genomic regions. In addition to predefined randomization and evaluation strategies, regioneR is fully customizable. Implements function to evaluate local specificity of detected association. Used for association analysis of genomic regions based on permutation tests.

• Resource
• RRID
• 4 months ago - submitted by Mitchell Cheung

DNA Sequencing facility provides Sanger sequencing and other services to labs on campus and beyond. Users can choose the degree of in-house processing to accommodate individual needs, providing flexibility for clients of all levels.

• Resource
• SciCrunch
• 4 months ago - submitted by Edyta Vieth

Facility supports micro- and nanoscale fabrication, microsensor development, and atomic-scale materials synthesis. Provides comprehensive technical capabilities, including microlithography (mask aligners, photoresist processing, laser lithography), thin film deposition (e-beam evaporation, sputtering, PECVD, atomic layer deposition), and advanced characterization tools (profilometry, spectroscopic ellipsometry). Fabrication processes are supported by both dry etching (DRIE, RIE) and wet chemical processing (RCA clean, KOH etch, solvent benches), as well as thermal processing systems such as diffusion furnaces and rapid thermal annealing.

• Resource
• RRID
• 4 months ago - by Anonymous

Core offers processing and testing of environmental DNA samples, assay development, field surveys and training services. The equipment housed in the center allows users to receive fluorescence-based qPCR analyses, for quantifying DNA concentration and abundances, and plate preparation.

• Resource
• RRID
• 4 months ago - by Anonymous

Facility is outfitted with the Aquatic Habitats for Accelerated Bioresearch (AHAB) fish rearing system (Aquatic Habitats, Apopka, FL). This system is composed of five-foot wide, five-level, stainless steel racks using any combination of tank sizes (3 and 10 liter). Water input lines deliver UV-sterilized water to each tank through an independent valve. The fish tanks have self-cleaning bottoms and the effluent system features an optically clear design. The filter system requires minimal maintenance, and maintains high water quality, dramatically reduced disease and mortality.

• Resource
• RRID
• 4 months ago - by Anonymous

Core provides animal care and assists investigators in fulfilling their obligation to plan and conduct animal research in accordance with scientific, humane and ethical principles. SARF has a multizone temperature and airflow control to allow for various environments necessary to research projects. SARF also features quarantine suite with separate entrance and personal protective equipment (PPE), and is outfitted with RAIR HD Super Mouse 750 ventilated rack holding 126 cages. Daily care and feeding is provided by trained staff, and surgery prep, surgical area, and recovery area are available for use.

• Resource
• RRID
• 4 months ago - by Anonymous

Core offers tools and technical expertise for research and training in microscopy with both light and electron microscopes. Maintains and operates electron microscopes (SEM with EDS), confocal laser scanning microscope, and tancillary equipment needed for preparation of specimens for EM and other microscopy. Light microscopes encompass optics for bright-field, phase-contrast, Nomarski differential-interference, polarizing, and fluorescence imaging.

• Resource
• RRID
• 4 months ago - by Anonymous

Core to maximize sharing of research platforms and expertise between research groups in the Department of Medicine and their collaborators. Provides research equipment and expertise to accelerate translation of laboratory discoveries into clinical applications to improve patient care. It supports translational research through advanced tools, expertise in biotechnology, biomolecular sciences, and the development of new treatments and biomarkers, aiming to advance first-in-person clinical trials.

• Resource
• RRID
• 4 months ago - by Anonymous