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Firefly luciferase is a protein with a molecular weight of approximately 61 kDa that catalyzes the oxidation of luciferin to oxyluciferin in the presence of ATP, magnesium ions, and oxygen, emitting bioluminescence at a wavelength of around 560 nm during the oxidation process. Renilla luciferase is a protein with a molecular weight of approximately 36 kDa that can catalyze the oxidation of coelenterazine to coelenteramide in the presence of oxygen, emitting bioluminescence at a wavelength of around 480 nm during the oxidation process. Both types of bioluminescence can be measured using a chemiluminescence instrument. Typically, the 5' UTR or promoter of the target gene is cloned upstream of the Firefly Luciferase, or the 3' UTR is cloned downstream of the Firefly Luciferase. The amount of firefly luciferase detected can be used to assess the transcriptional regulatory effects of the promoter or regulatory elements. Renilla luciferase serves as an internal control to eliminate variations in cell number, transfection efficiency, and other factors. The Dual Luciferase Reporter Gene Assay Kit first measures the activity of the firefly luciferase reporter gene using luciferin as the substrate, and then measures the activity of the Renilla luciferase reporter gene using coelenterazine as the substrate while quenching the firefly luciferase reaction. This kit is characterized by its high sensitivity.

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• 2 years ago - submitted by Yujing Zhong

DAPI is a fluorescent dye commonly used in molecular biology and cell biology research. DAPI is known for its ability to bind to DNA, specifically to the minor groove of double-stranded DNA. This dye emits blue fluorescence when excited by ultraviolet light, allowing researchers to visualize and stain DNA in various biological samples. Hoechst dyes are DNA-specific fluorescent stains that bind to the minor groove of double-stranded DNA. They emit blue fluorescence when excited by ultraviolet light. These dyes are widely used for staining DNA in various biological samples. The FluoroPure™-grade of Hoechst and DAPI powders are manufactured using stringent purification processes to minimize impurities that could interfere with fluorescence imaging. This ensures that the DAPI and Hoechst provides reliable and consistent results with minimal background noise. The >98% purity of FluoroPure grade reagents make them particularly suitable for demanding applications such as super-resolution microscopy and live-cell imaging, where precise and accurate visualization of DNA is essential.

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• 2 years ago - submitted by Yujing Zhong

Detergent, equivalent to Nonidet P-40.Triton™ X-100 is used as a non-ionic detergent, emulsifier and dispersing agent. It is useful for isolation of membrane proteins. It is also used in electrophoresis. It is an active ingredient in influenza vaccine (Fluzone). It finds applications in the dispersion of carbon materials for soft composite materials and restricting colony expansion in Aspergillus nidulans in microbiology. Further, it is an active ingredient in homemade vinyl record cleaning fluids together with distilled water and isopropyl alcohol. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

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• 2 years ago - submitted by Yujing Zhong

triple-label multiplex immunofluorescence kit，YOBIBIO Biosciences, #YB006

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• 2 years ago - submitted by Yujing Zhong

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-mouse IgG and anti-rabbit IgG affinity-purified, highly cross-adsorbed secondary antibodies.

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• 2 years ago - submitted by Yujing Zhong

enhanced chemiluminescence detection kit，Tiangen Biotech, #PA112

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• 2 years ago - submitted by Yujing Zhong

enhanced chemiluminescence detection kit, Tiangen Biotech, #PA112

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• 2 years ago - by Anonymous

The Pierce Dilution-Free Rapid Gold BCA Protein Assay Kit is an effortless, fast, and accurate assay optimized to determine total protein concentration. It is detergent-compatible and has a 5X wider dynamic range than traditional protein assays of 0.02–10 mg/mL. This wide dynamic range eliminates the need to dilute most samples to get them into range of the assay.

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• 2 years ago - submitted by Yujing Zhong

Halt™ Protease Inhibitor Cocktail (100X), #87786

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• 2 years ago - submitted by Yujing Zhong

Taq Pro Universal SYBR qPCR Master Mix

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• 2 years ago - submitted by Yujing Zhong

Taq Pro Universal SYBR qPCR Master Mix

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• 2 years ago - submitted by Yujing Zhong

HiScript III RT SuperMix for qPCR (+gDNA wiper)

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• 2 years ago - submitted by Yujing Zhong

FastPureRNA Isolation Kit

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• 2 years ago - submitted by Yujing Zhong

CytoFLEX flow cytometer

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• 2 years ago - submitted by Yujing Zhong

Zombie Aqua™ is an amine-reactive fluorescent dye that is non-permeant to live cells but permeant to cells with compromised membranes. Thus, it can be used to assess live vs. dead status of mammalian cells. Zombie Aqua™ is a polar, water-soluble dye providing very bright green fluorescence, making it suitable for use in multi-color detection.

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• 2 years ago - submitted by Yujing Zhong

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.

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• 2 years ago - submitted by Yujing Zhong

Falcon® 100 µm cell strainer is a sterile, easy-to-use device for rapidly isolating primary cells to consistently obtain a uniform single-cell suspension from tissues. Applications include stem cells and primary cells. Individually packaged, 50/case

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• 2 years ago - submitted by Yujing Zhong

Benchtop instrument for semi-automated dissociation of tissues into single-cell suspensions or thorough homogenates. Single sample or two samples in parallel can be processed. Two types of unique gentleMACS Tubes used with instrument enable time-saving and easy dissociation or homogenization of tissues in closed system. Instrument offers optimized gentleMACS Programs for variety of specific applications. Special protocols have been developed for various tissues

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• 2 years ago - submitted by Yujing Zhong

The C1.18.4 monoclonal antibody is ideal for use as a non-reactive isotype-matched control for mouse IgG2a antibodies in most in vivo and in vitro applications.

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• 2 years ago - submitted by Yujing Zhong

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

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• 2 years ago - submitted by Yujing Zhong