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Unique stand-alone platform and voice-controlled DNA/protein gel workstation for gel imaging.
enhanced chemiluminescence detection kit,Tiangen Biotech, #PA112
enhanced chemiluminescence detection kit, Tiangen Biotech, #PA112
The Pierce Dilution-Free Rapid Gold BCA Protein Assay Kit is an effortless, fast, and accurate assay optimized to determine total protein concentration. It is detergent-compatible and has a 5X wider dynamic range than traditional protein assays of 0.02–10 mg/mL. This wide dynamic range eliminates the need to dilute most samples to get them into range of the assay.
Halt™ Protease Inhibitor Cocktail (100X), #87786
Taq Pro Universal SYBR qPCR Master Mix
Taq Pro Universal SYBR qPCR Master Mix
HiScript III RT SuperMix for qPCR (+gDNA wiper)
FastPureRNA Isolation Kit
Software can use data files exported in FCS 2.0 or 3.0 formats, providing analysis capability for major BD instrument platforms that use BD FACSuite, BD FACSDiva, BD FACSArray, BD Accuri C6, or BD CellQuest Pro software.
CytoFLEX flow cytometer
Zombie Aqua™ is an amine-reactive fluorescent dye that is non-permeant to live cells but permeant to cells with compromised membranes. Thus, it can be used to assess live vs. dead status of mammalian cells. Zombie Aqua™ is a polar, water-soluble dye providing very bright green fluorescence, making it suitable for use in multi-color detection.
CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.
Falcon® 100 µm cell strainer is a sterile, easy-to-use device for rapidly isolating primary cells to consistently obtain a uniform single-cell suspension from tissues. Applications include stem cells and primary cells. Individually packaged, 50/case
Benchtop instrument for semi-automated dissociation of tissues into single-cell suspensions or thorough homogenates. Single sample or two samples in parallel can be processed. Two types of unique gentleMACS Tubes used with instrument enable time-saving and easy dissociation or homogenization of tissues in closed system. Instrument offers optimized gentleMACS Programs for variety of specific applications. Special protocols have been developed for various tissues
The C1.18.4 monoclonal antibody is ideal for use as a non-reactive isotype-matched control for mouse IgG2a antibodies in most in vivo and in vitro applications.
Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.
Ultra-Low Attachment surface with covalently bonded hydrogel that minimizes cell attachment, protein absorption, enzyme activation and cellular activation The surface is noncytotoxic, biologically inert and nondegradable Round bottoms with 330 µL total volume Recommended working volumes of 75 to 200 µL Nonreversable lids with condensation rings to reduce contamination Sterilized by gamma radiation and nonpyrogenic Individual alphanumeric codes for well identification Individually wrapped
The Foxp3 / Transcription Factor Staining Buffer Set has been formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as FOXP3 and Ki-67, as well as cytokines and chemokines.
Brefeldin A (BFA) is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.