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This 1X Red Blood Cell (RBC) Lysis Buffer is formulated to optimally lyse red blood cells in single-cell suspensions from hematopoietic tissues of mice, such as the spleen, and human peripheral blood. This buffer contains ammonium chloride, and when used according to the specified procedure, it effectively lyses red blood cells while minimizing the impact on lymphocytes. Nucleated red blood cells are not effectively lysed by ammonium chloride

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• 2 years ago - submitted by Yujing Zhong

The Applied Biosystems QuantStudio real-time PCR system can detect extremely small changes in gene expression, as little as 1.5-fold, which might be overlooked by other systems. Its dynamic range of up to 10 logs allows for tracking a wide range of expression changes across various sample types and conditions.

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• 2 years ago - submitted by Yujing Zhong

FLUOstar® Omega is a multi-mode microplate reader with three detection modes. It utilizes ultra-fast UV/Visible spectroscopy or filters, along with high-sensitivity filters for all other detection modes. The FLUOstar Omega is an ideal microplate reader for applications in the life sciences field.

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• 2 years ago - submitted by Yujing Zhong

Mesothelin, also known as MSLN or MPF, is a protein encoded by the MSLN gene in humans. This gene is located at 16p13.3. It encodes a precursor protein that is cleaved into two products: megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor is a cytokine that stimulates the formation of megakaryocyte colonies in the bone marrow. Mesothelin is a glycosylphosphatidylinositol-anchored cell surface protein that acts as a cell adhesion protein. This protein is overexpressed in epithelial mesothelioma, ovarian cancer, and certain squamous cell carcinomas. This kit is intended for in vitro qualitative or quantitative analysis and is for research use only, not for clinical diagnostics. It is suitable for serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids.

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• 2 years ago - submitted by Yujing Zhong

The Steriflip filter unit is designed for use with 50 mL centrifuge tubes, eliminating the need for transferring samples. To filter, simply connect the 50 mL centrifuge tube containing the sample to the Steriflip unit, invert it, and apply suction. Once the filtration is complete, the filtrate is collected in the 50 mL centrifuge tube attached below.

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• 2 years ago - submitted by Yujing Zhong

The Crystal Violet Staining Solution produced by BiYunTian is a commonly used staining solution for tissue or cell staining that can color cell nuclei deep purple. Crystal violet is a basic dye that can bind to the DNA in cell nuclei, resulting in nuclear staining. A 100 ml package of this staining solution can stain at least 200 samples, while a 500 ml package can stain at least 1,000 samples.

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• 2 years ago - submitted by Yujing Zhong

SeaPlaque® Agarose, 25 g, is the original low melting temperature agarose.

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• 2 years ago - submitted by Yujing Zhong

The BioTek Synergy HTX is a compact, economical multimode reader for 6- to 384-well microplates and Take3 microvolume plates. The unique dual optics design provides superior performance for UV-Vis absorbance, fluorescence, luminescence, and AlphaScreen/AlphaLISA workflows. With incubation and shaking, plus a dual reagent injector module, the system will meet all your assay requirements now and in the future. Synergy HTX is controlled by the easy-to-use, yet powerful, Gen5 software for data collection, analysis, exporting, and reporting.

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• 2 years ago - submitted by Yujing Zhong

Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing Dojindo’s highly water-soluble tetrazolium salt. WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. CCK-8 is a one-bottle solution; no premixing of components is required. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells.

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• 2 years ago - submitted by Yujing Zhong

suitable for release of physiologically active rat hepatocytes, Type IV, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

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• 2 years ago - submitted by Yujing Zhong

TruSeq RNA Sample Preparation Kit

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• 2 years ago - submitted by Yujing Zhong

This enzyme randomly cleaves single-stranded or double-stranded DNA to produce deoxyribonuclease endonuclease with 5'-P terminal oligonucleotides. The enzyme has been purified to remove proteases and nucleases, which enhances its stability in the neutral pH range, making it suitable for RNA preparation. The main features of TRIzol reagent include: The ability to isolate RNA, DNA, and protein from the same sample. Excellent lysis capability even for difficult-to-handle sample types. Formulation and protocol optimization for tissues, cells, serum, viruses, and bacteria. Reliable purification of RNA from different sample sizes and sources TRIzol reagent performs well when processing small amounts of tissue (50–100 mg) and cells (5 × 10^6), as well as large amounts of tissue (≥1 g) and cells (>10^7). The provided protocols can be used for purifying samples from human, animal, plant, or bacterial sources. TRIzol reagent maintains RNA integrity due to its efficient inhibition of ribonuclease activity while disrupting cells and lysing cellular components during sample homogenization. The ease of use of TRIzol reagent allows for the simultaneous processing of multiple samples, with the entire process taking less than one hour. Total RNA isolated using TRIzol reagent is free from protein and DNA contamination. Formulation allows for multiple separations of various molecular targets TRIzol reagent enables the sequential precipitation of RNA, DNA, and protein from a single sample. After homogenizing the sample with TRIzol reagent, chloroform is added to separate the homogenate into a clear upper aqueous phase (containing RNA), an interphase, and a red lower organic phase (containing DNA and protein). RNA is precipitated from the aqueous phase using isopropanol, while DNA is precipitated from the interphase/organic phase using ethanol. Protein is precipitated from the phenol-ethanol supernatant using isopropanol. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for downstream applications.

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• 2 years ago - submitted by Yujing Zhong

TRIzol reagent is a complete, ready-to-use reagent that isolates high-quality total RNA or simultaneously separates RNA, DNA, and protein from various biological samples. This phenol and guanidine isothiocyanate monophasic solution can isolate RNA, DNA, and protein components from cell and tissue samples of humans, animals, plants, yeast, or bacteria within one hour. The main features of TRIzol reagent include: The ability to isolate RNA, DNA, and protein from the same sample. Excellent lysis capability even for difficult-to-handle sample types. Formulation and protocol optimization for tissues, cells, serum, viruses, and bacteria. Reliable purification of RNA from different sample sizes and sources TRIzol reagent performs well when processing small amounts of tissue (50–100 mg) and cells (5 × 10^6), as well as large amounts of tissue (≥1 g) and cells (>10^7). The provided protocols can be used for purifying samples from human, animal, plant, or bacterial sources. TRIzol reagent maintains RNA integrity due to its efficient inhibition of ribonuclease activity while disrupting cells and lysing cellular components during sample homogenization. The ease of use of TRIzol reagent allows for the simultaneous processing of multiple samples, with the entire process taking less than one hour. Total RNA isolated using TRIzol reagent is free from protein and DNA contamination. Formulation allows for multiple separations of various molecular targets TRIzol reagent enables the sequential precipitation of RNA, DNA, and protein from a single sample. After homogenizing the sample with TRIzol reagent, chloroform is added to separate the homogenate into a clear upper aqueous phase (containing RNA), an interphase, and a red lower organic phase (containing DNA and protein). RNA is precipitated from the aqueous phase using isopropanol, while DNA is precipitated from the interphase/organic phase using ethanol. Protein is precipitated from the phenol-ethanol supernatant using isopropanol. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for downstream applications.

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• 2 years ago - submitted by Yujing Zhong

Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for various cell culture applications, including washing cells before dissociation, transporting cell and tissue samples, diluting cells during counting, and preparing reagents. Formulations that contain calcium and magnesium are generally used as transport media or for reagent preparation.

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• 2 years ago - submitted by Yujing Zhong

D-Luciferin is a commonly used substrate for luciferase, widely utilized across the biotechnology field, particularly in in vivo imaging techniques. Its mechanism of action involves the oxidation of luciferin (the substrate) to produce light in the presence of ATP and luciferase. When luciferin is in excess, the number of emitted photons is positively correlated with the concentration of luciferase. After transfecting a plasmid encoding the luciferase gene (Luc) into cells and introducing it into research animals such as mice and rats, luciferin is injected, and bioluminescence imaging (BLI) is used to detect changes in light intensity. This allows for real-time monitoring of disease progression or the therapeutic effects of drugs. The influence of ATP on this reaction system can also be utilized, where changes in bioluminescence intensity indicate energy levels or vital signs.

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• 2 years ago - submitted by Yujing Zhong

Bupivacaine is a NMDA receptor inhibitor. Bupivacaine can block sodium, L-calcium, and potassium channels.Bupivacaine potently blocks SCN5A channels with the IC50 of 69.5 μM. Bupivacaine can be used for the research of chronic pain.

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• 2 years ago - submitted by Yujing Zhong

Basement membrane is a layer of matrix membrane located at the basal surface of epithelial cells in animals. OrganoGel is a matrix gel formed from basement membrane components extracted from mouse tumor tissue. The main components of this matrix gel include laminin, collagen IV, and heparan sulfate proteoglycans (Kleinman et al. 1986). Additionally, this matrix gel contains various growth factors, such as epidermal growth factor (EGF), platelet-derived growth factor (PDGF), nerve growth factor (NGF), basic fibroblast growth factor (FGF-2), transforming growth factor beta (TGF-beta), and insulin-like growth factor (IGF) (Vukicevic et al. 1992). Product Characteristics: This product is in liquid form at 4°C but becomes gel-like when heated to 37°C. Once the matrix gel has solidified, it can be returned to 4°C overnight, and the matrix gel can be re-liquefied.

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• 2 years ago - submitted by Yujing Zhong

Carprofen is a nonsteroid anti-inflammatory agent, acts as a multi-target FAAH/COX inhibitor, with IC50s of 3.9 μM, 22.3 μM and 78.6 μM for COX-2, COX-1 and FAAH, respectively.

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• 2 years ago - submitted by Yujing Zhong

Carprofen is a nonsteroid anti-inflammatory agent, acts as a multi-target FAAH/COX inhibitor, with IC50s of 3.9 μM, 22.3 μM and 78.6 μM for COX-2, COX-1 and FAAH, respectively.

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• 2 years ago - submitted by Yujing Zhong

Firefly luciferase is a protein with a molecular weight of approximately 61 kDa that catalyzes the oxidation of luciferin to oxyluciferin in the presence of ATP, magnesium ions, and oxygen, emitting bioluminescence at a wavelength of around 560 nm during the oxidation process. Renilla luciferase is a protein with a molecular weight of approximately 36 kDa that can catalyze the oxidation of coelenterazine to coelenteramide in the presence of oxygen, emitting bioluminescence at a wavelength of around 480 nm during the oxidation process. Both types of bioluminescence can be measured using a chemiluminescence instrument. Typically, the 5' UTR or promoter of the target gene is cloned upstream of the Firefly Luciferase, or the 3' UTR is cloned downstream of the Firefly Luciferase. The amount of firefly luciferase detected can be used to assess the transcriptional regulatory effects of the promoter or regulatory elements. Renilla luciferase serves as an internal control to eliminate variations in cell number, transfection efficiency, and other factors. The Dual Luciferase Reporter Gene Assay Kit first measures the activity of the firefly luciferase reporter gene using luciferin as the substrate, and then measures the activity of the Renilla luciferase reporter gene using coelenterazine as the substrate while quenching the firefly luciferase reaction. This kit is characterized by its high sensitivity.

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• 2 years ago - submitted by Yujing Zhong