Curator Dashboard

This is the description of your tool/resource, which will be shown on the Tool/Resource Summary page, in search results, etc. It should not be as comprehensive and as formal as the Tool/Resource Purpose and Summary description (step 3), so please feel free to use concise and catchy wording. Maximum length is 1023 characters. This description may be changed once the tool/resource has been registered.

• Resource
• NIF
• 1 year ago - by Anonymous

Total RNA was isolated from tissues and cells, including fat-rich tissues and difficult-to-extract samples.

• Resource
• RRID
• 1 year ago - submitted by scott qiu

TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube. After special optimization, this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR. dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent cross contamination.

• Resource
• RRID
• 1 year ago - submitted by scott qiu

By lysis of cells, release of RNA, removal of proteins, DNA and other impurities, high purity RNA products are finally obtained. The commonly used traditional method is guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, and most plant materials.

• Resource
• RRID
• 1 year ago - submitted by scott qiu

Real time PCR commercially available kit

• Resource
• RRID
• 1 year ago - submitted by Nicole Reddy

DNA extraction kit

• Resource
• RRID
• 1 year ago - submitted by Nicole Reddy

Used in the ddPCR reaction

• Resource
• RRID
• 1 year ago - submitted by Nicole Reddy

Used in the ddPCR reaction

• Resource
• RRID
• 1 year ago - submitted by Nicole Reddy

Used in the ddPCR reaction

• Resource
• RRID
• 1 year ago - submitted by Nicole Reddy

This digital PCR supermix delivers maximum PCR efficiency and sensitivity for the amplification and detection of DNA targets using hydrolysis probe–based assays.

• Resource
• RRID
• 1 year ago - submitted by Nicole Reddy

ddPCR Analysis tool

• Resource
• RRID
• 1 year ago - submitted by Nicole Reddy

Triple-labeling Multiplex Immunofluorescence Kit

• Resource
• RRID
• 2 years ago - submitted by Yujing Zhong

Disodium clodronate is a selective macrophage-killing agent that removes macrophages from tissues by inducing apoptosis. Currently, the ideal reagent on the market for efficient macrophage depletion is disodium clodronate liposomes. The clodronate molecules are encapsulated within liposomal vesicles, offering high activity, enhanced stability, and ease of use. Intravenous injection of 0.2 mL clodronate liposomes can achieve an 80-90% clearance rate of macrophages in the spleen (red pulp macrophages) within 24 hours. A version of this product without disodium clodronate is also available, with the liposomes containing only PBS, which can be used as a control in experiments to determine if macrophage depletion is specifically due to the effects of clodronate liposome injection.

• Resource
• RRID
• 2 years ago - submitted by Yujing Zhong

Clodronate Liposomes were developed by Professor Nico van Rooijen at Vrije University Amsterdam, Netherlands. They utilize the phagocytic mechanism of macrophages to deliver the membrane-impermeable clodronate into cells. Within macrophages, clodronate dissolved in the aqueous phase of the liposomes is released under the action of lysosomal phosphatases and accumulates inside the cell. When clodronate reaches a certain concentration, it induces apoptosis in macrophages, thereby achieving the goal of macrophage depletion. Clodronate Liposomes are suitable for various injection routes, including intravenous, intraperitoneal, subcutaneous, intranasal, and testicular injections. The injection dosage depends on the mouse’s body weight, injection schedule, route of administration, and the specific experimental objectives.

• Resource
• RRID
• 2 years ago - submitted by Yujing Zhong

Zombie Aqua™ is an amine-reactive fluorescent dye that is non-permeant to live cells but permeant to cells with compromised membranes. Thus, it can be used to assess live vs. dead status of mammalian cells. Zombie Aqua™ is a polar, water-soluble dye providing very bright green fluorescence, making it suitable for use in multi-color detection.

• Resource
• RRID
• 2 years ago - submitted by Yujing Zhong

for nucleic acid staining

• Resource
• RRID
• 2 years ago - submitted by Yujing Zhong

The Thermo Scientific PageRuler Prestained Protein Ladder is a protein size marker for SDS-PAGE and Western blotting, consisting of a mixture of ten proteins (10 to 180 kDa) that are prestained in blue, orange, and green. The protein ladder is provided in a ready-to-use format and can be directly loaded onto gels without the need for heating, reduction, or the addition of sample buffer prior to use.

• Resource
• RRID
• 2 years ago - submitted by Yujing Zhong

a Wnt pathway agonist

• Resource
• RRID
• 2 years ago - submitted by Yujing Zhong

a Wnt pathway inhibitor

• Resource
• RRID
• 2 years ago - submitted by Yujing Zhong

NF-kB pathway inhibitor

• Resource
• RRID
• 2 years ago - submitted by Yujing Zhong