By now, only limited information on the Roseobacter group thriving at the seafloor is available. Hence, the current study was conducted to determine their abundance and diversity within Pacific sediments along the 180° meridian. We hypothesize a distinct biogeographical distribution of benthic members of the Roseobacter group linked to nutrient availability within the sediments and productivity of the water column. Lowest cell numbers were counted at the edge of the south Pacific gyre and within the north Pacific gyre followed by an increase to the north with maximum values in the highly productive Bering Sea. Specific quantification of the Roseobacter group revealed on average a relative abundance of 1.7 and 6.3% as determined by catalyzed reported deposition-fluorescence in situ hybridization (CARD-FISH) and quantitative PCR (qPCR), respectively. Corresponding Illumina tag sequencing of 16S rRNA genes and 16S rRNA transcripts showed different compositions containing on average 0.7 and 0.9% Roseobacter-affiliated OTUs of the DNA- and RNA-based communities. These OTUs were mainly assigned to uncultured members of the Roseobacter group. Among those with cultured representatives, Sedimentitalea and Sulfitobacter made up the largest proportions. The different oceanic provinces with low nutrient content such as both ocean gyres were characterized by specific communities of the Roseobacter group, distinct from those of the more productive Pacific subarctic region and the Bering Sea. However, linking the community structure to specific metabolic processes at the seafloor is hampered by the dominance of so-far uncultured members of the Roseobacter group, indicating a diversity that has yet to be explored.

Information on environmental conditions shaping archaeal communities thriving at the seafloor of the central Pacific Ocean is limited. The present study was conducted to investigate the diversity, composition, and function of both entire and potentially active archaeal communities within Pacific deep-sea sediments. For this purpose, sediment samples were taken along the 180° meridian of the central Pacific Ocean. Community composition and diversity were assessed by Illumina tag sequencing targeting archaeal 16S rRNA genes and transcripts. Archaeal communities were dominated by Candidatus Nitrosopumilus (Thaumarchaeota) and other members of the Nitrosopumilaceae (Thaumarchaeota), but higher relative abundances of the Marine Group II (Euryarchaeota) were observed in the active compared to the entire archaeal community. The composition of the entire and the active archaeal communities was strongly linked to primary production (chlorophyll content), explaining more than 40% of the variance. Furthermore, we found a strong correlation of the entire archaeal community composition to latitude and silicic acid content, while the active community was significantly correlated with primary production and ferric oxide content. We predicted functional profiles from 16S rRNA data to assess archaeal community functions. Latitude was significantly correlated with functional profiles of the entire community, whereas those of the active community were significantly correlated with nitrate and chlorophyll content. The results of the present study provide first insights into benthic archaeal communities in the Pacific Ocean and environmental conditions shaping their diversity, distribution, and function. Additionally, they might serve as a template for further studies investigating archaea colonizing deep-sea sediments.