The Thick-billed Flowerpecker (Dicaeum agile), a small member of the flowerpecker family, inhabits tropical Southern Asia, and is widely distributed from India east to Indonesia and Timor. In this study, next generation sequencing (NGS) was used to obtain a mitochondrial genome sequence for D. agile. The complete mitogenome was 16,809bp in length, with a GC content of 46.40%. The genome sequence contained thirteen protein-coding genes, one 12S RNA gene, one 16S RNA gene, and 22 tRNA genes. A phylogenetic tree constructed for the family confirmed that D. agile is closely related to another species of Dicaeum (Dicaeum eximium). The mitochondrial genome of the Thick-billed Flowerpecker will be useful for future phylogeographic studies of this species.

The plain flowerpecker, Dicaeum concolor, is a tiny bird classified in the flowerpecker family Dicaeidae, and is an important pollinator and disperser of mistletoes in forests. This species is distributed across South and Southeast Asia. For further phylogeographic studies of D. concolor, the complete mitochondrial genome was sequenced here. The genome (GenBank accession no. MW429430) is 16,796 bp in length and comprises 13 protein-coding genes, 22 transfer RNAs, and two ribosomal RNAs. The GC content of the genome sequence is 45.46%, with an overall base composition of 30.76% A, 30.92% C, 14.53% G, and 23.79% T. A maximum-likelihood analysis placed D. concolor as sister relationship to D. eximium. The mitochondrial genome of the plain flowerpecker will be useful for studies of molecular evolution in flowerpeckers.

The yellow-vented flowerpecker (Dicaeum chrysorrheum Temminck & Laugier) is a small bird in the Dicaeidae family. To explore the phylogenetic relationships within the genus Dicaeum, we sequenced the complete mitochondrial genome of D. chrysorrheum using a next generation sequencing platform. The mitogenome of D. chrysorrheum (GenBank accession number, MW629121) has a length of 16,818 bp, with a nucleotide composition of 30.62% A, 23.45% T, 31.34% C, and 14.59% G. The genome is comprised of 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes. The total length of the protein-coding genes is 11,402 bp, accounting for 67.8% of the total length of the mitochondrial genome. Of the 13 protein-coding genes, 10 have ATG start codons and three genes terminate with incomplete stop codons. A maximum-likelihood phylogenetic tree of 16 bird species placed D. chrysorrheum and D. agile as sister to the other three Dicaeum species (D. concolor, D. eximium and D. cruentatum). This new mitogenome will be useful for further phylogenetic studies of the genus Dicaeum.

Dicaeum (family Dicaeidae) is a genus of passerines distributed in tropical southern Asia and Australasia. The scarlet-backed flowerpecker (Dicaeum cruentatum Linnaeus) is a member of this genus found in China, India, and many Southeast Asian countries. To contribute to phylogenetic studies of Dicaeum, the complete mitochondrial genome of D. cruentatum was sequenced using a next-generation sequencing platform. The mitogenome of D. cruentatum (GenBank accession number MW629122) was 16,812 bp in length with an overall base composition of 30.97% A, 23.67% T, 30.28% C, and 14.07% G. The whole genome contained 13 protein-coding genes (PCGs), two ribosomal RNAs (rRNAs), and 22 transfer RNAs (tRNAs). Almost all PCGs had an ATN start codon except COX1 which started with GTG. Most of the PCGs terminated with a complete stop codon, but ND2, COX3, and ND4 had incomplete stop codons. The 16S rRNA was 1599 bp in length, while 12S rRNA was 986 bp. The Dicaeum species showed monophyletic clustering in the ML phylogeny and shows D. cruentatum as a sister taxa of D. eximium and D. concolor. This new mitogenome will provide genomic resources for future phylogenetic studies in passerines.