The outbreak of food-borne pathogens has become a serious concern; therefore, the detection of pathogenic bacteria in food is required. Untreated, sensitive, and reliable sensors should be developed for the detection of Staphylococcus aureus (S. aureus). In this study, a sensitive antibody-based electrochemical immunosensor was developed using antibody (Ab)-hierarchical mesoporous silica (HMS) bio-conjugates for label-free detection of low concentrations of S. aureus. First, a bio-template method based on butterfly wings was used to prepare the HMS. Then, the carrier material was amino-functionalized to cross-link the antibody with glutaraldehyde. The Ab-HMS bio-conjugates were then immobilized on a glassy carbon electrode (GCE), and the presence of S. aureus was detected by analyzing the changes in the peak currents after the antigen-antibody complex formation. Differential pulse voltammetry (DPV) was performed with bacterial concentrations ranging from 10 to 2 × 103 colony forming units (CFU) mL-1. Selective tests were performed using Escherichia coli (E. coli), Listeria monocytogenes (L. monocytohenes), and Salmonella, and the selective assays showed specific detection of S. aureus using the sensor. In addition, the immunosensor showed a good linear relationship between the peak current increase and logarithmic S. aureus concentration (R 2 = 0.9759) with a fast detection time (20 min) and detection limit of 11 CFU mL-1. When the electrochemical impedance spectroscopy (EIS) was performed under the same conditions, the results showed a good linear relationship between the impedance change value and the bacterial concentration (R 2 = 0.9720), the limit of detection (LOD) was 12 CFU mL-1. The performance of the sensor was compared with that of the colony counting method in the spiked milk sample test. The results showed no significant difference in the test results. Hence, this electrochemical immunosensor can be used to quickly detect S. aureus in actual food samples with a high sensitivity, specificity and stability.

A simple and sensitive Mn-ZnS quantum dot room-temperature phosphorescent immunosensor for detecting microcystin-LR was developed. This sensor adopted antigens and antibodies as recognition units and used Mn-ZnS RTP QDs as sensing materials to specifically bind with MC-LR. The structurally specific binding between the microcystin-LR antibody and MC-LR led to the aggregation of antibody-crosslinked QDs, and then the electrons of QDs would be transferred to the complex, leading to the phosphorescence quenching of QDs. The microcystin-LR antigen-antibody specific binding site was first analyzed. This phosphorescent immunosensor rapidly and sensitively detected microcystin-LR, with linear ranges of 0.2-1.5 μg L-1 and 1.5-20 μg L-1 and a detection limit of up to 0.024 μg L-1. Meanwhile, coexisting pollutants of microcystin-LR in water did not significantly interfere with microcystin-LR detection. The new sensor was applied to detect real water samples and showed high sensitivity and selectivity.

Sensitive and reliable detection of tumour markers is of great significance for early diagnosis and monitoring recurrence of cancers. Herein, a simple electrochemical immunosensor is developed with an integrated electrochemical probe on the sensing surface, which is able to sensitively and reagentlessly detect the breast cancer biomarker, human epidermal growth factor receptor 2 (ErbB2). Ferrocene (Fc) is chosen as the signal indicator and covalently grafted on cationic polyelectrolyte poly(ethylene imine) (Fc-PEI). The redox Fc-PEI could alternately assemble with carboxyl functionalized single-walled carbon nanotubes (SWNTs) on an indium tin oxide electrode through layer-by-layerelectrostatic assembly. After Anti-ErbB2 antibody is covalently immobilized onto the outermost SWNTs layer followed by blocking the electrode with bovine serum albumin, a sensing interface with recognitive probe and electrochemical probe is obtained. In the presence of ErbB2, the formed antigen-antibody complex makes a barrier to inhibit electro-transfer of inner Fc, leading to a decreased electrochemical response. Owing to the SWNTs-facilitated charge transfer and abundant surface-bound probes, the developed sensor demonstrates outstanding performance for reagentless detection of ErbB2 in terms of wide detection range (1.0-200.0 ng mL-1) and low detection limit (0.22 ng mL-1). The developed immunosensor also exhibits good selectivity, reproducibility and stability. Real analysis of ErbB2 in human serum samples is also demonstrated.