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PeptideAtlas provides a multi-organism, publicly accessible compendium of peptides identified in a large set of tandem mass spectrometry proteomics experiments collected for human, mouse, yeast, and several other organisms.

(last updated: Nov 28, 2017)

Data or Model Molecule

319 Results - per page

AccessionSample TitleSummaryOrganismCharacteristicsInstrumentTreatmentGrowthDigestionExtractSeparationReference
PAe000829HS1_0505_NeutrophilHS1_0505_NeutrophilLTQ
PAe000798H293b-2nd-c-500ugH293b-2nd-c-500ugLTQ
PAe001721Nuc_Hs_Tcell_Nuc6Nuc_Hs_Tcell_Nuc6LTQPMID:17519225
PAe000076cdc15_cdc23_oldICATS. cerevisiae cdc15 vs cdc23 mutant comparison. This was the first experiment done in this series. The older ICAT reagent was used.LTQ
PAe000811H293b-b-200ugH293b-b-200ugLTQ
PAe000821COPD7_1105_CD4COPD7_1105_CD4; Check detail in Sample Description.LTQ
PAe000851H293CoCl2-b1-200ugH293CoCl2-b1-200ugLTQ
PAe000352Li_OGE1_3-10_LTQLi_OGE1_3-10_LTQLTQ
PAe000858HS6_1105_CD4HS6_1105_CD4LTQ
PAe000116Human Erythroleukemia K562 cell line,QHuman huamn erythroleukemia K5632 cell line I think...HumanK562 (erythroleukemia)LTQK562 grown in suspension.Proteins in the pooled fractions were alkylated with iodoacetamide, desalted into 100 mM NH₄HCO₃, digested, lyophilized, and processed in the same manner as sample 1 (referring PSM1018) and sample 2 (referring PSM1019).Cells were washed twice by centrifugation, and pellets were flash frozen in liquid nitrogen. Cell pellets were suspended in lysis buffer (140mM potassium phosphate, pH 7.4, 150mM NaF, 1mM Na₃VO₄, 6mM EDTA, 6mM EGTA, 250mM NaCl, 4mM DTT) containing 40µg/mL leupeptin, 5µg/mL pepstatin A, 4mM benzamidine, and 20mM PMSF and sonicated 4 × 15 s at 4°C (Branson, microtip probe). Lysates were centrifuged at 200000g for 30 min at 4°C and soluble proteins recovered in the supernatant. Typically 10⁸ cells yielded ~15mg of protein.Proteins were homogenized in lysis buffer on a gel filtration column (Sephacryl HR300 (26/60), Amersham) equilibrated in lysis buffer. The column was run at 1.3 mL/min, collecting 8-mL fractions over the included volume.PMID:15228325
PAe000866H293b-2nd-a-400ugH293b-2nd-a-400ugLTQ
PAe000853H293-b-200ugH293-b-200ugLTQ
PAe000818HS4_0805_CD8HS4_0805_CD8LTQ
PAe000805HS4_0805_CD4HS4_0805_CD4LTQ
PAe000831HS1_0505_CD4HS1_0505_CD4LTQ
PAe001647HsBrain2_PSHsBrain2_PSLTQ
PAe001646HsBrain1_PEHsBrain1_PELTQ
PAe00086103-H293CoCl2-40uL03-H293CoCl2-40uLLTQ
PAe001734PM_Hs_Tcell_PlasmaMembrane1PM_Hs_Tcell_PlasmaMembrane1LTQPMID:17519225
PAe000808HS3_0805_MonocyteHS3_0805_MonocyteLTQ
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