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PeptideAtlas provides a multi-organism, publicly accessible compendium of peptides identified in a large set of tandem mass spectrometry proteomics experiments collected for human, mouse, yeast, and several other organisms.
(last updated: Nov 28, 2017)
Data or Model Molecule| Accession | Sample Title | Summary | Organism | Characteristics | Instrument | Treatment | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| PAe001361 | Kc-Wnt-a-dsh836_exp_082 | Kc-Wnt-a-dsh836_exp_082 Kc cells | LTQ | PMID:17450130 | |||||||
| PAe001357 | Dm_S2_membrane_exp_024 | Dm_S2_membrane_exp_024 Hypotonic lysis of S2 cells. -Amount used for experiment: 2mg -Labeling buffer used: 1% RapiGestheavy ICAT only used for labeling -Enzyme: Trypsin-Separation: SCX (60 fractions) | LTQ | PMID:17450130 | |||||||
| PAe001374 | Dm_Lar_HL_exp_043 | Dm_Lar_HL_exp_043 Larvae from yw flies were pricked using a tungsten needle and and centrifuged into a eppendorf containing siliconized glass wool and frozen immediately. Total proteins were precipitated using ice-cold acetone and dissolved in labelling buffer (6M Urea, 50mM Tris/HCl pH 8.3, 0.05% SDS, 5mM EDTA). No ICAT labelling was done and these samples were purified using seppak clean up. | LTQ | PMID:17450130 | |||||||
| PAe001375 | Dm_S2_Nuclear_FFE_exp_053 | Dm_S2_Nuclear_FFE_exp_053 Hypotonic lysis of S2 cells, Nuclear fraction prepared by high salt extraction -Labeling buffer used: 6M Urea, 50mM Tris/HCl pH 8.3, 0.05% SDS, 5mM EDTA -Enzyme: Trypsin - Peptide FFE - No modification of cysteines | LTQ | PMID:17450130 | |||||||
| PAe001376 | Dm_Kc_Gel_exp_066 | Dm_Kc_Gel_exp_066 Gelfiltration was done on a Superose 6 column (HR 10/30, Amersham-Pharmacia, Switzerland) controlled by an automatic fast protein liquid chromatography (FPLC) station (Amersham-Pharmacia) at room temperature. The column was equilibrated with a native buffer containing 150 mM NaCl, 1 mM DTT, 0.2 mM sodium-vanadate, 5mM EDTA, 1 g/ml Aprotinin (Sigma-Aldrich, Germany), and 50 mM Tris pH 7.2. The column was calibrated with Catalase (232 kDa), Albumine (67 kDa), Ovalbumin (43 kDa), Chymotrypsinogen (25 kDa), Ribonuclease A (15.6 kDa; all Amersham-Pharmacia), and Cytidin (Merk, Switzerland). Before loading onto the column cytosolic fractions were cleared of nucleic acids by precipitation with polyethyleneimine (Sigma-Aldrich, 0.1% w/v). 200 l of the cleared cytosolic fraction (1 mg/ml protein) was loaded onto the column and eluted at a flow rate of 0.3 ml/minute. Fractions of 0.6 ml calculated to be < 25 kDa were collected. The eluate was precipitated by trichloroacetic acid (50%), the pelle | LTQ | PMID:17450130 | |||||||
| PAe001378 | Dm_Lar_membrane_exp_038 | Dm_Lar_membrane_exp_038 Total larval extract prepared in RIPA buffer. 2-3g of the acetone precipitated extract was resuspended in 5mM TrisHCl, pH8.0 Overlayed with 48% sucrose, 28.5% Sucrose, 10% Sucrose and spun in an ultracentrifuge @21'000rpm in aTHSA64 rotor for 3h30min @ 4erlayed with 48% sucrose, 28.5% Sucrose, 10% Sucremoved diluted 1:1 with dH2O and pelleted by ultracentrifugation @21'000rpm in aTHSA64 rotor for 2h @ 4 28.5% Sucrose, 10% Sucrd 1x with dH2O (no resuspension).The pellet was resuspended in 1% RapiGest, 50mM Tris-HCl pH8.3, EDTA 5mM.The membranes (5-10 mg total) were labeled 1:1 using cleavable ICAT. | LTQ | PMID:17450130 | |||||||
| PAe001379 | Dm_Adult_Heads_Cytoplasm_exp_044 | Dm_Adult_Heads_Cytoplasm_exp_044 Adult: heads, cytoplasmic fractions (soluble proteins) -Enzyme: Trypsin1:1 labeling using cleavable ICAT -Separation: SCX (60 fractions) -Avidin colums was used. -Sample validation: The head fraction was inspected for contaminants through the microscope and contaminating parts were removed manually.-SepPack cleanup was done | LTQ | PMID:17450130 | |||||||
| PAe001380 | Dm_Lar_IPG_exp_057 | Dm_Lar_IPG_exp_057 2 mg Proteins from yw larvae extracted by RIPA bufferTryptic digestRan on peptide IPG strips 3.4-4.8 (non liner gradient , 22cm, Amersham Pharmacia) | LTQ | PMID:17450130 | |||||||
| PAe001382 | Dm_Adult_Heads_Membrane_exp_025 | Dm_Adult_Heads_Membrane_exp_025 -Flies were frozen in liquid nitrogen and the heads were isolated by sieving. -Sample validation: The head fraction was inspected for contaminants through the microscope and contaminating parts were removed manually. Then hypotonic lysis as for the S2 | LTQ | PMID:17450130 | |||||||
| PAe001383 | Dm_Lar_FB_suc_exp_027 | Dm_Lar_FB_suc_exp_027 Fatbodies were extracted by ultracentrifugation from 3rd Instar larvae -20% Sucrose treated ICAT-labeled using (heavy) normal (untreated) ICAT-labeled (light) -Amount used for experiment: 2mg per sample -Labeling buffer used: 8M Urea, 50mM Tris/HCl pH 8.3, 0.25% SDS, 5mM EDTA -Enzyme: Trypsin -Separation: SCX (60 fractions)Avidin column usedSepPack purification used | LTQ | PMID:17450130 | |||||||
| PAe001384 | Dm_Emb_2hAEL_exp_030 | Dm_Emb_2hAEL_exp_030 embryos were collected for 20 minutes and aged for 1hour 30 minutes.Total extract from these embryos -Amount used for experiment: 2mg -Labeling buffer used: 6M Urea, 50mM Tris/HCl pH 8.3, 0.05% SDS, 5mM EDTA -Enzyme: Trypsin -Separation: SCX (60 fractions) -Avidin colums was used.-SepPack cleanup was done | LTQ | PMID:17450130 | |||||||
| PAe001385 | Dm_Kc_N-Glyc_exp_041 | Dm_Kc_N-Glyc_exp_041 Drosophila | LTQ | PMID:17450130 | |||||||
| PAe001386 | Dm_Lar_FB_rap_exp_028 | Dm_Lar_FB_rap_exp_028 Fatbodies were extracted by ultracentrifugation from 3rd Instar larvae -Rapamycin-treated ICAT-labeled using (light) -normal (untreated) ICAT-labeled (heavy) -Amount used for experiment: 2mg per sample -Labeling buffer used: 8M Urea, 50mM Tris/HCl pH 8.3, 0.25% SDS, 5mM EDTA -Enzyme: Trypsin -Separation: SCX (60 fractions)Avidin column usedSepPack purification used | LTQ | PMID:17450130 | |||||||
| PAe001388 | Dm_Emb_0-24hAEL_exp_055 | Dm_Emb_0-24hAEL_exp_055 embryos were collected 0-24h AEL.Total extractCarbamido-methylation of cys | LTQ | PMID:17450130 | |||||||
| PAe001343 | Kc-Wnt-a-wg2026_exp_079 | Kc-Wnt-a-wg2026_exp_079 Kc cells | LTQ | PMID:17450130 | |||||||
| PAe001340 | Dm_S2_cytoplasmic_exp_032 | Dm_S2_cytoplasmic_exp_032 Hypotonic lysis of S2 cells. -Amount used for experiment: 2mg -Labeling buffer used: 6M Urea, 50mM Tris/HCl pH 8.3, 0.05% SDS, 5mM EDTA -Enzyme: Trypsin -Separation: SCX (60 fractions)-Avidin columns was used. | LTQ | PMID:17450130 | |||||||
| PAe001341 | Dm_Adult_HL_exp_029 | Dm_Adult_HL_exp_029 Flies were punched with a needle and centrifuged on a glasswool cushion.The haemolymph (clear liquid) was collected at the bottom of the tube.60 SCX fractions; ICAT labelingAvidin usedSepPack cleanup | LTQ | PMID:17450130 | |||||||
| PAe001342 | Dm_Kc_exp_068 | Dm_Kc_exp_068 Kc cells | LTQ | PMID:17450130 | |||||||
| PAe001344 | Dm_Adult_Heads_Cytoplasm_exp_015 | Dm_Adult_Heads_Cytoplasm_exp_015 -Strain: yw -Enzyme: Trypsin1:1 labeling using cleavable ICAT -Separation: SCX (60 fractions) -Avidin colums was used. -Sample validation: The head fraction was inspected for contaminants through the microscope and contaminating parts were removed manua | LTQ | PMID:17450130 | |||||||
| PAe001347 | Dm_Lar_FFE_Protein_exp_061 | Dm_Lar_FFE_Protein_exp_061 Whole proetin extracts were prepared using RIPA buffer from 3rd Instar larvae -Amount used for experiment: 25mg per sample -Labeling buffer used: 1% Rapigest -No trypsin used -ICAT labelled-FFE - pH 4-7 | LTQ | PMID:17450130 |
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