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This study aimed to investigate the role of a novel duck miR-290, which targets Phosphatidyqinositol-3 kinase (PI3K), in regulating melanogenesis in Liancheng white ducks. Differentially expressed miRNAs (DEmiRNA) associated with melanogenesis were first screened by transcriptome sequencing of mouth skin and skin from 130-day-old Liancheng white ducks. The interaction between novel miR-290 and the PI3K 3'UTR was verified by a dual-luciferase reporter assay. Primary duck melanocytes were then isolated from the mouth skin tissue, and the function of miR-290 was examined at the cellular level. The results revealed that novel miR-290 directly targeted PI3K, and the levels of p-PI3K and p-AKT were significantly decreased in the mouth skin compared with skin (P < 0.05), whereas p-GSK3β, Integrin-linked kinase (ILK), Microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), and Melanocortin 1 Receptor (MC1R) were significantly increased in the mouth skin(P < 0.05). The isolated melanocytes were spindle-shaped and positive for DOPA and melanocytic markers.Overexpression of miR-290 significantly promoted melanocyte proliferation and melanin production (P < 0.01);(RT-qPCR) and Western blot analysis revealed that miR-290 inhibited the expression of PI3K and protein kinase B (AKT) (P < 0.05) and increased the expression of ILK, Glycogen Synthase Kinase 3β (GSK3β), MC1R, MITF, TYR, tyrosinase-related protein 1 (TYRP1), and tyrosinase-related protein 2 (TYRP2) (P < 0.05). In conclusion, this study identifies a novel duck miR-290 as a positive regulator of melanogenesis, which promotes melanocyte proliferation and melanin synthesis in Liancheng white ducks by suppressing PI3K/AKT signaling and activating downstream melanogenic genes.
Pubmed ID: 41380317
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Computable knowledge regarding functions of genes and gene products. GO resources include biomedical ontologies that cover molecular domains of all life forms as well as extensive compilations of gene product annotations to these ontologies that provide largely species-neutral, comprehensive statements about what gene products do. Used to standardize representation of gene and gene product attributes across species and databases.
View all literature mentionsSoftware tool for finding the minimum free energy hybridization of a long and a short RNA. The hybridization is performed in a kind of domain mode, i.e., the short sequence is hybridized to the best fitting part of the long one. The tool is primarily meant as a means for microRNA target prediction.
View all literature mentionsCatalogs of predicted microRNA targets in worm (based on ce6 genome assembly), fly (dm3), mouse (mm9) and human (hg18). We follow standard seed parameter settings and consider seeds of length 6-8 bases, beginning at position 2 of the microRNA. No mismatches or loops are allowed, but a single G:U wobble is allowed in 7- or 8-mers. In genes missing a 3' UTR annotation, 500 bp (fly), 800 bp (human and mouse) or 300 bp (worm) downstream of the annotated end of the coding sequence were used as the predicted UTR. For each organism, a catalog with zero flank and with a flank of 3 and 15 bases upstream and downstream.
View all literature mentionsIntegrated database resource consisting of 16 main databases, broadly categorized into systems information, genomic information, and chemical information. In particular, gene catalogs in completely sequenced genomes are linked to higher-level systemic functions of cell, organism, and ecosystem. Analysis tools are also available. KEGG may be used as reference knowledge base for biological interpretation of large-scale datasets generated by sequencing and other high-throughput experimental technologies.
View all literature mentionsA commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.
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