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Bud blight caused by Pseudomonas syringae is a serious disease affecting tea plants and causing severe damage to production output and quality. Phages play an important role in controlling the development of bacterial diseases in plants. Previous studies have shown that the tolerance of phage-treated tea plants to bud blight was notably greater compared with that of the control group. In the present study, we determined the effect of bacteriophage therapy on physiological and biochemical parameters of tea leaves. Transmission electron microscopy (TEM) was used to analyze the cellular structure of tea leaves, and bioinformatics was used to analyze the phage. Results revealed that bacteriophage treatment can enhance the expression of antioxidant enzyme genes (CsSOD, CsCAT, and CsPOD). The levels of osmotic adjustment compounds, including proline and soluble sugars, were also elevated, suggesting that bacteriophage enhances the osmotic adjustment capacity in tea plants. TEM analysis revealed that the integrity of the cell structure of the tea leaves treated with phage was notably better compared with that of the control group. Interestingly, we also observed that the phage lysed the animal pathogen Salmonella as well as the plant pathogen P. syringae. Using NCBI BLASTn to compare the entire genome with other nucleotide sequences, we found that the phage LDT325 exhibited cross-species characteristics that had not been previously reported. In summary, our findings demonstrate that bacteriophages can protect tea plants from damage caused by bacterial diseases by regulating antioxidant systems.
Pubmed ID: 39850129
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NIH genetic sequence database that provides annotated collection of all publicly available DNA sequences for almost 280 000 formally described species (Jan 2014) .These sequences are obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects, including whole-genome shotgun (WGS) and environmental sampling projects. Most submissions are made using web-based BankIt or standalone Sequin programs, and GenBank staff assigns accession numbers upon data receipt. It is part of International Nucleotide Sequence Database Collaboration and daily data exchange with European Nucleotide Archive (ENA) and DNA Data Bank of Japan (DDBJ) ensures worldwide coverage. GenBank is accessible through NCBI Entrez retrieval system, which integrates data from major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of GenBank database are available by FTP.
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