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An optimum study on the laser scanning confocal microscopy techniques for BiFC assay using plant protoplast.

Jinhong Yuan | Daiyu Li | Yi Liang | Yao Meng | Li Li | Lin Yang | Mingyue Pei | Liuchun Feng | Junhua Li
Botanical studies | 2024

The bimolecular fluorescence complementation (BiFC) assay is commonly used for investigating protein-protein interactions. While several BiFC detection systems have been developed, there is a limited amount of research focused on using laser scanning confocal microscope (LSCM) techniques to observe protoplasts. Protoplasts are more susceptible to damage and instability compared to their original cell state due to the preparation treatments they undergo, which makes it challenging for researchers to manipulate them during observation under LSCMs. Therefore, it is crucial to utilize microscope techniques properly and efficiently in BiFC assays.

Pubmed ID: 38194078

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Associated grants

  • Agency: National Natural Science Foundation of China,
    Id: 31970380
  • Agency: National Natural Science Foundation of China,
    Id: U1504319
  • Agency: National Natural Science Foundation of China,
    Id: 31670317
  • Agency: "Chunhui Program" Collaborative Scientific Research Project of the Ministry of Education of China,
    Id: 202200157

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SPSS (tool)

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Software package used for interactive, or batched, statistical analysis in social science, health sciences and marketing. Software platform offers advanced statistical analysis, a library of machine-learning algorithms, text analysis, open-source extensibility, integration with big data and deployment into applications.Versions that were produced by SPSS Inc. before the IBM acquisition (Versions 18 and earlier) would be given origin or publisher of SPSS Inc. in Chicago.

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SigmaPlot (tool)

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Statistical analysis and scientific graphing software for Windows OS.

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