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Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping.

Yang Liu | Marcello DiStasio | Graham Su | Hiromitsu Asashima | Archibald Enninful | Xiaoyu Qin | Yanxiang Deng | Pino Bordignon | Marco Cassano | Mary Tomayko | Mina Xu | Stephanie Halene | Joseph E Craft | David Hafler | Rong Fan
Research square | 2022

We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 198 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 283 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.

Pubmed ID: 35378748

Research resources used in this publication

None found

Antibodies used in this publication

None found

Associated grants

  • Agency: NIMH NIH HHS, United States
    Id: RF1 MH128876
  • Agency: NCI NIH HHS, United States
    Id: UG3 CA257393
  • Agency: NIDDK NIH HHS, United States
    Id: U54 DK106857
  • Agency: NIA NIH HHS, United States
    Id: U54 AG076043
  • Agency: NCI NIH HHS, United States
    Id: R01 CA245313

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This is a list of tools and resources that we have found mentioned in this publication.


STAR (tool)

RRID:SCR_004463

Software performing alignment of high-throughput RNA-seq data. Aligns RNA-seq reads to reference genome using uncompressed suffix arrays.

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Miltenyi Biotec (tool)

RRID:SCR_008984

An Organization portal, Antibody supplier, Service resource,

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ggplot2 (tool)

RRID:SCR_014601

Open source software package for statistical programming language R to create plots based on grammar of graphics. Used for data visualization to break up graphs into semantic components such as scales and layers.

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BioAnalyzer 2100 (tool)

RRID:SCR_019715

2100 Bioanalyzer system is an established automated electrophoresis tool for the sample quality control of biomolecules. The 2100 Bioanalyzer instrument, together with the 2100 Expert Software and Bioanalyzer assays, provide highly precise analytical evaluation of various samples types in many workflows, including next generation sequencing (NGS), gene expression, biopharmaceutical, and gene editing research. Digital data is provided in a timely manner and delivers objective assessment of sizing, quantitation, integrity and purity from DNA, RNA, and proteins. Minimal sample volumes are required for an accurate result, and the data may be exported in a many different formats for ease-of-use.

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