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Large-scale generation of megakaryocytes from human embryonic stem cells using transgene-free and stepwise defined suspension culture conditions.

Bowen Zhang | Xumin Wu | Guicheng Zi | Lijuan He | Sihan Wang | Lin Chen | Zeng Fan | Xue Nan | Jiafei Xi | Wen Yue | Lei Wang | Liu Wang | Jie Hao | Xuetao Pei | Yanhua Li
Cell proliferation | 2021

Ex vivo engineered production of megakaryocytes (MKs) and platelets (PLTs) from human pluripotent stem cells is an alternative approach to solve shortage of donor-donated PLTs in clinics and to provide induced PLTs for transfusion. However, low production yields are observed and the generation of clinically applicable MKs and PLTs from human pluripotent stem cells without genetic modifications still needs to be improved.

Pubmed ID: 33615584

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FlowJo (tool)

RRID:SCR_008520

Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.

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