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Effect of the macroalgae Asparagopsis taxiformis on methane production and rumen microbiome assemblage.

Breanna Michell Roque | Charles Garrett Brooke | Joshua Ladau | Tamsen Polley | Lyndsey Jean Marsh | Negeen Najafi | Pramod Pandey | Latika Singh | Robert Kinley | Joan King Salwen | Emiley Eloe-Fadrosh | Ermias Kebreab | Matthias Hess
Animal microbiome | 2019

Recent studies using batch-fermentation suggest that the red macroalgae Asparagopsis taxiformis has the potential to reduce methane (CH4) production from beef cattle by up to ~ 99% when added to Rhodes grass hay; a common feed in the Australian beef industry. These experiments have shown significant reductions in CH4 without compromising other fermentation parameters (i.e. volatile fatty acid production) with A. taxiformis organic matter (OM) inclusion rates of up to 5%. In the study presented here, A. taxiformis was evaluated for its ability to reduce methane production from dairy cattle fed a mixed ration widely utilized in California, the largest milk producing state in the US.

Pubmed ID: 33499933

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This is a list of tools and resources that we have found mentioned in this publication.


Greengenes (tool)

RRID:SCR_002830

Database that provides access to the current and comprehensive 16S rRNA gene sequence alignment for browsing, blasting, probing, and downloading. The data and tools can assist the researcher in choosing phylogenetically specific probes, interpreting microarray results, and aligning/annotating novel sequences. The 16S rRNA gene database provides chimera screening, standard alignment, and taxonomic classification using multiple published taxonomies. ARB users can use Greengenes to update local databases.

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RRID:SCR_006423

High quality ribosomal RNA databases providing comprehensive, quality checked and regularly updated datasets of aligned small (16S/18S, SSU) and large subunit (23S/28S, LSU) ribosomal RNA (rRNA) sequences for all three domains of life (Bacteria, Archaea and Eukarya). Supplementary services include a rRNA gene aligner, online tools for probe and primer evaluation and optimized browsing, searching and downloading on the website. The extensively curated SILVA taxonomy and the new non-redundant SILVA datasets provide an ideal reference for high-throughput classification of data from next-generation sequencing approaches. Alignment tool, SINA, is available for download as well as available for use online.

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mothur (tool)

RRID:SCR_011947

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RRID:SCR_013517

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VSEARCH (tool)

RRID:SCR_024494

Software versatile open source tool for metagenomics. Used for processing and preparing metagenomics, genomics and population genomics nucleotide sequence data.

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