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Osteosarcoma (OS) is a rare, insidious tumor of mesenchymal origin that most often affects children, adolescents, and young adults. While the primary tumor can be controlled with chemotherapy and surgery, it is the lung metastases that are eventually fatal. Multiple studies into the initial drivers of OS development have been undertaken, but few of these have examined innate immune/inflammatory signaling. A central figure in inflammatory signaling is the innate immune/stress-activated kinase double-stranded RNA-dependent protein kinase (PKR). To characterize the role of PKR in OS, U2OS, and SaOS-2 osteosarcoma cell lines were stably transfected with wild-type or dominant-negative (DN) PKR. Overexpression of PKR enhanced colony formation in soft agar (U2OS and SaOS-2), enhanced cellular migration (U2OS), and invasive migration (SaOS-2). In contrast, overexpression of DN-PKR inhibited attachment-independent growth, migration and/or invasion. These data demonstrate a role for inflammatory signaling in OS formation and migration/invasion and suggest the status of PKR expression/activation may have prognostic value.
Pubmed ID: 31240713
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This unknown targets
View all literature mentionsThis monoclonal targets RelB (C1E4) Rabbit mAb
View all literature mentionsThis monoclonal targets PACT
View all literature mentionsThis polyclonal targets c-Rel
View all literature mentionsThis monoclonal targets IkappaBbeta (7B4) Mouse mAb
View all literature mentionsThis monoclonal targets NF-KappaB p65, phospho (Ser536)
View all literature mentionsThis polyclonal targets Human TRBP
View all literature mentionsThis monoclonal targets Dnajc3
View all literature mentionsThis polyclonal targets EIF2S1
View all literature mentionsThis polyclonal targets phospho-PKR (Thr451)
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis unknown targets IkappaB-alpha
View all literature mentionsThis monoclonal targets beta-Tubulin I antibody produced in mouse
View all literature mentionsThis polyclonal targets Phospho-eIF2alpha (Ser51)
View all literature mentionsThis monoclonal targets NF-κB1
View all literature mentionsThis monoclonal targets NF-kappaB2 p100/p52
View all literature mentionsThis polyclonal targets Human EIF2AK2
View all literature mentionsThis polyclonal targets c-Myc
View all literature mentionsCell line U2OS is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line NIH 3T3 is a Spontaneously immortalized cell line with a species of origin Mus musculus
View all literature mentionsCell line SaOS-2 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HT-1080 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HOS is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MG-63 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsDigital image processing system where microscope settings and processing steps may be adjusted in single user interface. Can acquire images from variety of cameras. Includes software package for capturing, archiving and preparing images for publication. Allows users to visualize and present images in several dimensions. Functionality of imaging toolbox expands constantly with wide range of different modules that are tailored to specific applications or microscope accessories. This resource is duplicated by SCR_018376
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