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Molecular data reveal a new species of Rhopalias Stiles & Hassall, 1898 (Digenea, Echinostomatidae) in the Common opossum, Didelphismarsupialis L. (Mammalia, Didelphidae) in the Yucatán Peninsula, Mexico.

Jorge López-Caballero | Rosario Mata-López | Gerardo Pérez-Ponce de León
ZooKeys | 2019

A new species of Rhopalias Stiles & Hassall, 1898 is described from the small intestine of the Common opossum, Didelphismarsupialis Linnaeus from the Yucatán Peninsula, Mexico. Rhopaliasoochi sp. nov. is morphologically very similar to the type species of the genus, Rhopaliascoronatus (Rudolphi, 1819) Stiles & Hassall 1898, a species widely distributed in opossums across Mexico. A molecular phylogenetic analysis using a mitochondrial gene (cox1), and the nuclear ribosomal internal transcribed spacer region (ITS1-5.8S-ITS2), of specimens of R.coronatus collected in several localities of Mexico revealed that those from the Yucatán Peninsula, originally recorded on morphological grounds as R.coronatus actually represented an independent genetic lineage. Maximum Likelihood and Bayesian Inference analyses were performed for each data set independently, and for the concatenated data set (ITS1-5.8S-ITS2 + cox1). All phylogenetic analyses showed that the specimens from Yucatán represented a monophyletic lineage, with high bootstrap support and Bayesian posterior probabilities. In addition, the genetic divergence estimated between R.oochi sp. nov. and two species of Rhopalias, R.coronatus, and R.macracanthus Chandler, 1932 that also occur in Mexican marsupials ranged between 7-8% and 16-17%, for cox1, and between 0.1-0.2% and 7% for the ITS region, respectively. The molecular evidence gathered in this study (reciprocal monophyly in both phylogenetic analyses, and estimated genetic divergence) suggested that the specimens found in the intestine of D.marsupialis originally reported as R.coronatus from Yucatán, actually represent a new species. Morphological evidence was found through light and scanning electron microscopy to support the species distinction based on molecular data.

Pubmed ID: 31239821

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GenBank (tool)

RRID:SCR_002760

NIH genetic sequence database that provides annotated collection of all publicly available DNA sequences for almost 280 000 formally described species (Jan 2014) .These sequences are obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects, including whole-genome shotgun (WGS) and environmental sampling projects. Most submissions are made using web-based BankIt or standalone Sequin programs, and GenBank staff assigns accession numbers upon data receipt. It is part of International Nucleotide Sequence Database Collaboration and daily data exchange with European Nucleotide Archive (ENA) and DNA Data Bank of Japan (DDBJ) ensures worldwide coverage. GenBank is accessible through NCBI Entrez retrieval system, which integrates data from major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of GenBank database are available by FTP.

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RAxML (tool)

RRID:SCR_006086

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FigTree (tool)

RRID:SCR_008515

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MrBayes (tool)

RRID:SCR_012067

THIS RESOURCE IS NO LONGER IN SERVICE.Documented on February 28,2023. Software program for Bayesian inference and model choice across a wide range of phylogenetic and evolutionary models.

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jModelTest (tool)

RRID:SCR_015244

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