Searching the Resource Information Network

Our searching services are busy right now. Please try again later

  • Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Publication

Antibody Conditioning Enables MHC-Mismatched Hematopoietic Stem Cell Transplants and Organ Graft Tolerance.

Benson M George | Kevin S Kao | Hye-Sook Kwon | Brenda J Velasco | Jessica Poyser | Angela Chen | Alan C Le | Akanksha Chhabra | Cassandra E Burnett | Devon Cajuste | Malachia Hoover | Kyle M Loh | Judith A Shizuru | Irving L Weissman
Cell stem cell | 2019

Hematopoietic cell transplantation can correct hematological and immunological disorders by replacing a diseased blood system with a healthy one, but this currently requires depleting a patient's existing hematopoietic system with toxic and non-specific chemotherapy, radiation, or both. Here we report an antibody-based conditioning protocol with reduced toxicity and enhanced specificity for robust hematopoietic stem cell (HSC) transplantation and engraftment in recipient mice. Host pre-treatment with six monoclonal antibodies targeting CD47, T cells, NK cells, and HSCs followed by donor HSC transplantation enabled stable hematopoietic system reconstitution in recipients with mismatches at half (haploidentical) or all major histocompatibility complex (MHC) genes. This approach allowed tolerance to heart tissue from HSC donor strains in haploidentical recipients, showing potential applications for solid organ transplantation without immune suppression. Fully mismatched chimeric mice developed antibody responses to nominal antigens, showing preserved functional immunity. These findings suggest approaches for transplanting immunologically mismatched HSCs and solid organs with limited toxicity.

Pubmed ID: 31204177

Research resources used in this publication

Additional research tools detected in this publication

None found

Antibodies used in this publication

Associated grants

  • Agency: NCI NIH HHS, United States
    Id: R35 CA220434
  • Agency: NCI NIH HHS, United States
    Id: T32 CA009302
  • Agency: NIH HHS, United States
    Id: DP5 OD024558
  • Agency: NIGMS NIH HHS, United States
    Id: T32 GM119995
  • Agency: NIDDK NIH HHS, United States
    Id: R01 DK115600

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), eFluor™ 450, eBioscience (antibody)

RRID:AB_1548788

This monoclonal targets Ly-6G/Ly-6C

View all literature mentions

CD45.1 Monoclonal Antibody (A20), FITC (antibody)

RRID:AB_2534248

This monoclonal targets CD45.1

View all literature mentions

CD4 Monoclonal Antibody (GK1.5), APC (antibody)

RRID:AB_11152647

This monoclonal targets CD4

View all literature mentions

InVivoPlus anti-mouse CD154 (CD40L) (antibody)

RRID:AB_1107601

This monoclonal targets CD40L (CD154)

View all literature mentions

CD34 Monoclonal Antibody (RAM34), FITC, eBioscience (antibody)

RRID:AB_465021

This monoclonal targets CD34

View all literature mentions

Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (antibody)

RRID:AB_143165

This polyclonal targets IgG (H+L)

View all literature mentions

PE anti-mouse CD3 (antibody)

RRID:AB_312662

This monoclonal targets CD3

View all literature mentions

Rabbit Anti-Human cardiac Troponin I Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_869982

This polyclonal targets Human cardiac Troponin I

View all literature mentions

APC anti-mouse TCR Vbeta6 (antibody)

RRID:AB_2564056

This monoclonal targets TCR Vbeta6

View all literature mentions

CD117 (c-Kit) Monoclonal Antibody (2B8), APC, eBioscience (antibody)

RRID:AB_469429

This monoclonal targets CD117 (c-Kit)

View all literature mentions

Anti-Mouse CD19 PE 500 ug Antibody (antibody)

RRID:AB_465579

This monoclonal targets CD19

View all literature mentions

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-Cyanine7, eBioscience (antibody)

RRID:AB_469669

This monoclonal targets Ly-6A/E (Sca-1)

View all literature mentions

anti-mouse CD4 (antibody)

RRID:AB_1107636

This monoclonal targets CD4

View all literature mentions

NK1.1 Monoclonal Antibody (PK136), PE-Cyanine7, eBioscience (antibody)

RRID:AB_469665

This monoclonal targets NK1.1

View all literature mentions

InVivoPlus anti-mouse CD8α (antibody)

RRID:AB_10950145

This monoclonal targets CD8α

View all literature mentions

InVivoPlus anti-human/mouse/rat CD47 (antibody)

RRID:AB_2687806

This monoclonal targets CD47

View all literature mentions

Pacific Blue(TM) anti-mouse CD150 (SLAM) (antibody)

RRID:AB_2187962

This monoclonal targets CD150

View all literature mentions

CD5 Monoclonal Antibody (53-7.3), PE (antibody)

RRID:AB_2539168

This monoclonal targets CD5

View all literature mentions

CD45.2 Monoclonal Antibody (104), PE (antibody)

RRID:AB_2534922

This monoclonal targets CD45.2

View all literature mentions

InVivoMAb anti-mouse CD122 (IL-2Rβ) (antibody)

RRID:AB_2687820

This monoclonal targets CD122 (IL-2Rβ)

View all literature mentions

CD8 alpha Monoclonal Antibody (53-6.7), FITC (antibody)

RRID:AB_11153636

This monoclonal targets CD8 alpha

View all literature mentions

CD8a Monoclonal Antibody (53-6.7), PE, eBioscience (antibody)

RRID:AB_465529

This monoclonal targets CD8a

View all literature mentions

InVivoMAb anti-mouse c-Kit (CD117) (antibody)

RRID:AB_2687818

This monoclonal targets c-Kit (CD117)

View all literature mentions

CB6F1/J (organism)

RRID:IMSR_JAX:100007

Mus musculus with name CB6F1/J from IMSR.

View all literature mentions

About

The Stimulating Peripheral Activity to Relieve Conditions (SPARC) effort is the result of National Institutes of Health’s (NIH) drive to map out the neural circuitry responsible for visceral control in higher vertebrates. The SPARC Anatomy Working Group (SAWG) is responsible for the integrity of anatomical knowledge in SPARC

Recent News Entries

Contact Us

Maryann Martone, Ph.D.

Department of Neurosciences, School of Medicine

University of California, San Diego

About SciCrunch | Privacy Policy | Terms of Service

SciCrunch