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Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis.

Chih-Jung Kuo | Jian Gao | Jian-Wen Huang | Tzu-Ping Ko | Chao Zhai | Lixin Ma | Weidong Liu | Longhai Dai | Yung-Fu Chang | Ter-Hsin Chen | Yumei Hu | Xuejing Yu | Rey-Ting Guo | Chun-Chi Chen
Biochimica et biophysica acta. General subjects | 2019

Alanine and proline-rich protein (Apa) is a secreted antigen of Mycobacterium spp. which involves in stimulating immune responses and adhering to host cells by binding to fibronectin (Fn). Here, we report the crystal structure of Apa from Mycobacterium tuberculosis (Mtb) and its Fn-binding characteristics.

Pubmed ID: 31175911

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Phaser (tool)

RRID:SCR_014219

Crystallographic software which solves structures using algorithms and automated rapid search calculations to perform molecular replacement and experimental phasing methods.

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KaleidaGraph (tool)

RRID:SCR_014980

Data analysis, graphing, and management application that allows users to import, manipulate, analyze data, and create customized plots. Plots include x-y probability, histogram, box, percentile, horizontal bar, stack bar, column, stack column, polar, and pie. Binned data can be exported to a histogram, step plot, or spike plot. KaleidaGraph works with Windows and Macintosh systems.

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