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Publication

A human embryonic stem cell reporter line for monitoring chemical-induced cardiotoxicity.

Su-Yi Tsai | Zaniar Ghazizadeh | Hou-Jun Wang | Sadaf Amin | Francis A Ortega | Zohreh Sadat Badieyan | Zi-Ting Hsu | Miriam Gordillo | Ritu Kumar | David J Christini | Todd Evans | Shuibing Chen
Cardiovascular research | 2020

Human embryonic stem cells (hESCs) can be used to generate scalable numbers of cardiomyocytes (CMs) for studying cardiac biology, disease modelling, drug screens, and potentially for regenerative therapies. A fluorescence-based reporter line will significantly enhance our capacities to visualize the derivation, survival, and function of hESC-derived CMs. Our goal was to develop a reporter cell line for real-time monitoring of live hESC-derived CMs.

Pubmed ID: 31173076

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Associated grants

  • Agency: NHLBI NIH HHS, United States
    Id: R35 HL135778

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

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pX330-U6-Chimeric_BB-CBh-hSpCas9 (tool)

RRID:Addgene_42230

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pGolden-Hyg (tool)

RRID:Addgene_51423

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The Stimulating Peripheral Activity to Relieve Conditions (SPARC) effort is the result of National Institutes of Health’s (NIH) drive to map out the neural circuitry responsible for visceral control in higher vertebrates. The SPARC Anatomy Working Group (SAWG) is responsible for the integrity of anatomical knowledge in SPARC

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