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The single cell eukaryotic protozoan Acanthamoeba castellanii exhibits a remarkable ability to switch from a vegetative trophozoite stage to a cystic form, in response to stressors. This phenotypic switch involves changes in gene expression and synthesis of the cell wall, which affects the ability of the organism to resist biocides and chemotherapeutic medicines. Given that encystation is a fundamental survival mechanism in the life cycle of A. castellanii, understanding of this process should have significant environmental and medical implications. In the present study, we investigated the mechanism of A. castellanii encystation using a novel phonon microscopy technique at the single cell level. Phonon microscopy is an emerging technique to image cells using laser-generated sub-optical wavelength phonons. This imaging modality can image with contrast underpinned by mechanical properties of cells at an optical or higher resolution. Our results show that the Brillouin frequency, a shift of the colour of light induced by phonons, evolves in three well defined frequency bands instead of a simple shift in frequency. These observations confirm previous results from literature and provide new insights into the capacity of A. castellanii cyst to react quickly in harsh environments.
Pubmed ID: 31143495
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