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Fluorescent transcriptional reporters are widely used as signaling reporters and biomarkers to monitor pathway activities and determine cell type identities. However, a large amount of dynamic information is lost due to the long half-life of the fluorescent proteins. To better detect dynamics, fluorescent transcriptional reporters can be destabilized to shorten their half-lives. However, applications of this approach in vivo are limited due to significant reduction of signal intensities. To overcome this limitation, we enhanced translation of a destabilized fluorescent protein and demonstrate the advantages of this approach by characterizing spatio-temporal changes of transcriptional activities in Drosophila. In addition, by combining a fast-folding destabilized fluorescent protein and a slow-folding long-lived fluorescent protein, we generated a dual-color transcriptional timer that provides spatio-temporal information about signaling pathway activities. Finally, we demonstrate the use of this transcriptional timer to identify new genes with dynamic expression patterns.
Pubmed ID: 31140975
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Multi paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.
View all literature mentionsNon-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
View all literature mentionsImaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsA commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.
View all literature mentionsA commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.
View all literature mentionsSoftware that eliminates poorly aligned positions and divergent regions of a DNA or protein alignment so that it becomes more suitable for phylogenetic analysis.
View all literature mentionsServes Drosophila research community by collecting and distributing DNA clones and vectors; collecting and distributing Drosophila cell lines; developing and testing genomics technologies for use in Drosophila and assisting members of the research community in their use.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=Act5C-GAL4}25FO1/CyO, y[+] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.1}wwk[MI03816-TG4.1]/SM6a from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.0}Ae2[MI07482-TG4.0]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMJ23380}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR38F06-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.0}fz[MI12643-TG4.0]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR27G12-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR51F08-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.2}CG7744[MI04719-TG4.2]/SM6a from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.2}anchor[MI05468-TG4.2]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.0}CG2082[MI06537-TG4.0]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.2}ZnT41F[MI04971-TG4.2] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00647}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.0}Bulli[MI03821-TG4.0]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=Ugt36A1-GAL4.K}43A, y[1] w[*] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.2}anchor[MI05468-TG4.2]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Piezo-GAL4.1.0}IIA; MKRS/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.0}Plc21C[MI01911-TG4.0] CG43755[MI01911-TG4.0-X] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.2}CG43980[MI06742-TG4.2]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.1}CG10283[MI02713-TG4.1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=GAL4-Mef2.R}3 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=Mip-GAL4.TH}2M from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.1}CG5521[MI06530-TG4.1]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01964}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.2}MESK2[MI01548-TG4.2]/SM6a from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=GAL4-per.BS}3 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+m*]=Ubi-GAL4.U}2/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=ppk-GAL4.G}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.0}CG14995[MI10302-TG4.0]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR29C07-GAL4}attP2/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mW.hs]=GawB}gcm[rA87.C]/CyO from BDSC.
View all literature mentionsDiscontinued
View all literature mentionsDrosophila melanogaster with name w[*]; wg[Sp-1]/CyO; ry[506] Sb[1] P{ry[+t7.2]=Delta2-3}99B/TM6B, Tb[+] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.0}fz[MI12643-TG4.0]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.1}igl[MI12785-TG4.1]/SM6a from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Piezo-GAL4.1.0}III from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR10G10-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.2}CG33964[MI12535-TG4.2] CG13175[MI12535-TG4.2-X] SmF[MI12535-TG4.2-X]/SM6a from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=ppk-GAL4.G}3 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=GAL4-Antp.P1.A}1, y[1] w[*]; wg[Sp-1]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.1}CG4467[MI09204-TG4.1]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.1}tutl[MI00290-TG4.1]/SM6a from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.0}CG34347[MI05743-TG4.0]/TM3, Sb[1] Ser[1] from BDSC.
View all literature mentions