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The actomyosin network is involved in crucial cellular processes including morphogenesis, cell adhesion, apoptosis, proliferation, differentiation, and collective cell migration in Drosophila, Caenorhabditiselegans, and mammals. Here, we demonstrate that Drosophila larval blood stem-like progenitors require actomyosin activity for their maintenance. Genetic loss of the actomyosin network from progenitors caused a decline in their number. Likewise, the progenitor population increased upon sustained actomyosin activation via phosphorylation by Rho-associated kinase. We show that actomyosin positively regulates larval blood progenitors by controlling the maintenance factor Cubitus interruptus (Ci). Overexpression of the maintenance signal via a constitutively activated construct (ci.HA) failed to sustain Ci-155 in the absence of actomyosin components like Zipper (zip) and Squash (sqh), thus favoring protein kinase A (PKA)-independent regulation of Ci activity. Furthermore, we demonstrate that a change in cortical actomyosin assembly mediated by DE-cadherin modulates Ci activity, thereby determining progenitor status. Thus, loss of cell adhesion and downstream actomyosin activity results in desensitization of the progenitors to Hh signaling, leading to their differentiation. Our data reveal how cell adhesion and the actomyosin network cooperate to influence patterning, morphogenesis, and maintenance of the hematopoietic stem-like progenitor pool in the developing Drosophila hematopoietic organ.
Pubmed ID: 31138608
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Imaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsServes Drosophila research community by collecting and distributing DNA clones and vectors; collecting and distributing Drosophila cell lines; developing and testing genomics technologies for use in Drosophila and assisting members of the research community in their use.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-smo.RNAi}2 P{UAS-smo.RNAi}8/CyO, P{Wee-P.ph0}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-FLAG-smo.act}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00693}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name Df(2L)b88f32/In(2L)Cy, In(2R)Cy, Duox[Cy] dpy[lvI] Adc[b-1] pr[1] Bl[1] L[4] speck[2] from BDSC.
View all literature mentionsExpresses dsRNA for RNAi of zip (FBgn0005634) under UAS control, TRiP. This is a legacy resource.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-Rok.CAT}7.1 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=AyGAL4}25/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name Smg5[34Eb-2] Adh[D] pr[1] cn[1]/CyO, Adh[nB] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-mCD8.mRFP.LG}10b from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=tubP-GAL80[ts]}20; TM2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS03009}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-2xEGFP}AH2 from BDSC.
View all literature mentionsCollects, maintains and distributes Drosophila melanogaster strains for research. Emphasis is placed on genetic tools that are useful to a broad range of investigations. These include basic stocks of flies used in genetic analysis such as marker, balancer, mapping, and transposon-tagging strains; mutant alleles of identified genes, including a large set of transposable element insertion alleles; defined sets of deficiencies and a variety of other chromosomal aberrations; engineered lines for somatic and germline clonal analysis; GAL4 and UAS lines for targeted gene expression; enhancer trap and lacZ-reporter strains with defined expression patterns for marking tissues; and a collection of transposon-induced lethal mutations.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-GFP.E2f1.1-230}32 P{w[+mC]=UAS-mRFP1.NLS.CycB.1-266}19/CyO, P{ry[+t7.2]=en1}wg[en11]; MKRS/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=hsFLP}22, w[*] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-ci.HA.m1-4}2; hh[IJ32], P{w[+mW.hs]=GAL4-prd.F}RG1/TM2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] cv[1] sqh[AX3]; P{w[+mC]=sqh-GFP.RLC}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=tubP-GAL80[ts]}2/TM2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF02363}attP2 from BDSC.
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