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Analytical validation of real-time quantitative PCR assays for optimum diagnosis of vivax malaria.

Natália Ketrin Almeida-de-Oliveira | Otacílio C Moreira | Aline Rosa de Lavigne | Leila Mendonça-Lima | Guilherme Loureiro Werneck | Cláudio Tadeu Daniel-Ribeiro | Maria de Fátima Ferreira-da-Cruz
Memorias do Instituto Oswaldo Cruz | 2019

The prompt diagnosis of plasmodial species for effective treatment prevents worsening of individual health and avoids transmission maintenance or even malaria reintroduction in areas where Plasmodium does not exist. Polymerase chain reaction (PCR) allows for the detection of parasites below the threshold of microscopic examination.

Pubmed ID: 30726341

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SigmaPlot (tool)

RRID:SCR_003210

Statistical analysis and scientific graphing software for Windows OS.

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QIAGEN (tool)

RRID:SCR_008539

A commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.

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