Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice.

The present study aimed to evaluate the antitumor effect of Scutellaria barbata polysaccharides (SBPS) in a hepatoma mouse model and examine the serum proteins involved in the tumorigenesis and SBPS treatment. A hepatoma model was established by the subcutaneous inoculation of murine hepatocellular carcinoma into Kunming mice. The treatment (once a day) lasted until the tumor weight in the model group was ~1 g (~7‑10 days after inoculation). The sera proteins from each group were then collected and subjected to two‑dimensional gel electrophoresis. Differentially expressed proteins were screened out and representatives were identified using matrix‑assisted laser desorption ionization time‑of‑flight mass spectrometry. SBPS treatment at different doses significantly inhibited hepatoma growth (all P<0.01 vs. model group). The comparative serum proteomics showed that pseudouridine synthase 1 and chain A of the signal recognition particle Alu RNA‑binding heterodimer (Srp9/14) were increased in the serum of the H22 hepatoma‑bearing mice, and both were reduced by SBPS treatment. Mitochondrial ribosomal protein L24 was absent from the serum of H22 hepatoma‑bearing mice, and was restored by SBPS treatment to approximately the normal level. Taken together, SBPS inhibited the growth of hepatic carcinoma in mice and affected serum proteomic profiling.

Pubmed ID: 30664217