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Background: The association of primary brain tumors with Single Nucleotide polymorphisms (SNPs) in genes of folate metabolising enzymes have been reported to vary among different ethnic population. Here, we have studied the association of SNPs of folate metabolizing genes with the primary brain tumors (glioma and meningioma) in North Indian population. Methods: SNPs of genes coding for folate metabolizing enzymes was carried out in 288 study population from North India [Glioma (n=108), Meningioma (n=76) and healthy-control (n=104)]. The allele-specific polymerase chain reaction (ARMS-PCR) was used to analyse the SNP A1298C of the MTHFR (Methylenetetrahydrofolate-reductase) and the SNP A66G of the methionine synthase reductase (MTRR) genes. The PCR-RLFP (Restriction Fragment Length Polymorphism) was used to analyse the SNP C677T of the Methylene tetrahydrofolate-reductase and the SNP A2756G of the methionine-synthase (MTR) genes. Serum homocysteine, vitamin B12 and folate levels were evaluated in controls/ patients serum using Chemiluminescence immunoassay and the levels were correlated with SNPs genotype. Results: The CC genotype of MTHFR A1298C was observed to have reduced risk of having meningioma than AA genotype (odd ratio=0.62, 95%CI 0.32-0.97, p=0.03). Similarly, the AG genotype of MTRR A66G showed reduced risk of glioma than AA genotype (odd ratio=0.56, 95%CI 0.32-0.97, p=0.039). Furthermore, in patients with AA genotype of MTR A2756G and CT genotype of MTHFR C677T showed higher serum homocysteine level than GG genotype (8.6 μmol/L, p=0.048) and CC genotype (11.2μmol/L, p=0.039) respectively. Conclusion: Our findings provide an insight into the risk association of SNPs in MTHFR A1298C and MTRR A66G genes with glioma/meningioma patients. Further studies are needed to evaluate their clinical implications.
Pubmed ID: 30583664
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Tool used to design PCR primers from DNA sequence - often in high-throughput genomics applications. It does everything from mispriming libraries to sequence quality data to the generation of internal oligos.
View all literature mentionsNational Brain Tumor Society (NBTS) is a nonprofit organization committed to finding a cure for brain tumors. We aggressively drive strategic research, advocate for public policies that meet the critical needs of the brain tumor community, and provide patient information. Headquartered in Watertown, Massachusetts, with offices in San Francisco, California and Wilmington, Delaware, we host activities throughout the United States. Formed in 2008 by the merger of two leading organizations that had served the brain tumor community, the National Brain Tumor Foundation and the Brain Tumor Society, the National Brain Tumor Society is now the largest brain tumor nonprofit organization in the country. Both legacy organizations had been formed in the 1980s by parents and other people who were committed to increasing both research funding and access to resources specific to brain tumors. In 2010, the Kelly Heinz-Grundner Foundation, a Delaware-based organization, joined NBTS as a wholly-owned subsidiary. Founded in 2005, after the death of Kelly Heinz-Grundner to a brain tumor, the group has contributed to NBTS''s efforts to pursue research and public policies that benefit the brain tumor community. NBTS grant programs are effective for academic researchers, inclusive of industry expertise, and promising for the patient community. All funding is open to both the domestic and international research communities. The Innovation Research Grant Program supports catalytic transformative projects that will significantly move the field forward. These may include out-of-the-box projects or research that is critical to move therapies down the pipeline. Research that represents an incremental advance is not considered innovative. NBTS will accept Innovation Letters of Intent throughout the year. Researchers in academic or industry labs and at all stages of their career may be funded through this program.
View all literature mentionsWeb search tool to find regions of similarity between biological sequences. Program compares nucleotide or protein sequences to sequence databases and calculates statistical significance. Used for identifying homologous sequences.
View all literature mentionsIt is used to compare a novel sequence with those contained in nucleotide and protein databases by aligning the novel sequence with previously characterized genes.
View all literature mentionsStatistical software for ROC curve analysis. MedCalc performs several statistical tests such as method comparison, method evaluation, reference intervals, and meta-analysis.
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