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Evaluation of Real-time PCR for Diagnosis of Post-Kala-azar Dermal Leishmaniasis in Endemic Foci of Bangladesh.

Prakash Ghosh | Md Golam Hasnain | Faria Hossain | Md Anik Ashfaq Khan | Rajashree Chowdhury | Khaledul Faisal | Moshtaq Ahmed Mural | James Baker | Rupen Nath | Debashis Ghosh | Shomik Maruf | Mohammad Sohel Shomik | Rashidul Haque | Greg Matlashewski | Shinjiro Hamano | Malcolm S Duthie | Dinesh Mondal
Open forum infectious diseases | 2018

Post-kala-azar dermal leishmaniasis (PKDL) is a sequel to visceral leishmaniasis (VL), which is found in VL-endemic countries including Bangladesh. Because of these enigmatic cases, the success of the National Kala-azar Elimination Program is under threat. To date, diagnostic methods for PKDL cases in endemic regions have been limited to clinical examination and rK39 test or microscopy, and a suitable and accurate alternative method is needed. In this study, we investigated the application of real-time polymerase chain reaction (PCR) as a potential method for diagnosis of PKDL in comparison with microscopy.

Pubmed ID: 30320150

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SPSS (tool)

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Software package used for interactive, or batched, statistical analysis in social science, health sciences and marketing. Software platform offers advanced statistical analysis, a library of machine-learning algorithms, text analysis, open-source extensibility, integration with big data and deployment into applications.Versions that were produced by SPSS Inc. before the IBM acquisition (Versions 18 and earlier) would be given origin or publisher of SPSS Inc. in Chicago.

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Applied Biosystems (tool)

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QIAGEN (tool)

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A commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.

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