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The mouse embryo has long been central to the study of mammalian development; however, elucidating the cell behaviors governing gastrulation and the formation of tissues and organs remains a fundamental challenge. A major obstacle is the lack of live imaging and image analysis technologies capable of systematically following cellular dynamics across the developing embryo. We developed a light-sheet microscope that adapts itself to the dramatic changes in size, shape, and optical properties of the post-implantation mouse embryo and captures its development from gastrulation to early organogenesis at the cellular level. We furthermore developed a computational framework for reconstructing long-term cell tracks, cell divisions, dynamic fate maps, and maps of tissue morphogenesis across the entire embryo. By jointly analyzing cellular dynamics in multiple embryos registered in space and time, we built a dynamic atlas of post-implantation mouse development that, together with our microscopy and computational methods, is provided as a resource. VIDEO ABSTRACT.
Pubmed ID: 30318151
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Dissemination of public datasets containing raw crystallography data (known as ''diffraction images''), along with rich metadata for search and persistent handles for citation in publications. Storage is federated, meaning the public index contains no data itself and merely points to data stored in external labs and institutions. TARDIS is scheduled to be replaced by the Bioscience Data Platform (https://www.nectar.org.au/bioscience-data-platform-tardis-cloud).
View all literature mentionsMus musculus with name B6;129S-Sox2tm2Hoch/J from IMSR.
View all literature mentionsMus musculus with name STOCK Tg(Gal-EGFP)HX109Gsat/Mmucd from MMRRC.
View all literature mentionsMus musculus with name STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato.-EGFP)Luo/J from IMSR.
View all literature mentionsMus musculus with name B6.129S1(SJL)-Nkx2-5tm2(cre)Rph/J from IMSR.
View all literature mentions