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Functional analysis of a novel parasitic nematode-specific protein of Setaria digitata larvae in Culex quinquefasciatus by siRNA mediated RNA interference.

Magalla Bastian Chalitha Lakmal Somarathne | Yasanthi Illika Nilmini Silva Gunawardene | Naduvilath Vishvanath Chandrasekharan | Arjuna Nisantha Bandara Ellepola | Ranil Samantha Dassanayake
Parasites & vectors | 2018

Functional analysis of animal parasitic nematode genes is often quite challenging due to the unavailability of standardised in vitro culture conditions and lack of adequate tools to manipulate these genes. Therefore, this study was undertaken to investigate the suitability of Culex quinquefasciatus, as an in vivo culture platform for Setaria digitata larvae and RNA interference (RNAi), as a post-transcriptional gene silencing tool to study the roles of a vital gene that encodes a novel parasitic nematode-specific protein (SDNP).

Pubmed ID: 30314510

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ImageJ (tool)

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Promega (tool)

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