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Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then, the livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Protein identification was carried out by matrix-assisted laser desorption/ionization time-of-flight spectrometry (MALDI-TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion.
Pubmed ID: 30296410
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The CONICET is the leading organization dedicated to the promotion of science and technology in Argentina. It operates in four major areas * agricultural sciences, engineering and materials * life sciences and health * natural sciences * Social Sciences and Humanities The National Scientific and Technical Research Council (Spanish: Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET) is an Argentine government agency which directs and co-ordinates most of the scientific and technical research done in universities and institutes. It was established on 5 February 1958 by a decree of the national government. Its first director was Medicine Nobel Prize Bernardo A. Houssay. Nowadays, it is governed by a board completely independent from the federal government. It funds scientific research in three basic ways. First, CONICET gives grants for collective work to research teams of well-recognized scientists of every discipline (including social sciences and humanities). Secondly, it has a roll of 6,500 researches and 2,500 technicians as employees in different categories, from investigador asistente to investigador principal. And thirdly, it grants scolarships for doctoral and post-doctoral studies to 8,500 young researches from Argentina and other countries. (Wikipedia)
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