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Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging.

Sadie J Burkhow | Nicole M Stephens | Yu Mei | Maria Emilia Dueñas | Daniel J Freppon | Geng Ding | Shea C Smith | Young-Jin Lee | Basil J Nikolau | Steven A Whitham | Emily A Smith
Plant methods | 2018

Reverse genetic strategies, such as virus-induced gene silencing, are powerful techniques to study gene function. Currently, there are few tools to study the spatial dependence of the consequences of gene silencing at the cellular level.

Pubmed ID: 29849743

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Bio-Rad Laboratories (tool)

RRID:SCR_008426

Commercial instrument and chemical vendor. Developer and manufacturer of specialized technological products for life science research and clinical diagnostics markets.

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MATLAB (tool)

RRID:SCR_001622

Multi paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.

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