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The contribution of glutathione (GSH) in combating environmental stress in plants has long been known. Previous reports have pointed to the involvement of GSH in inducing various heat shock proteins (HSPs), but the molecular mechanism is yet to be explored. Here, we investigate how GSH induces the expression of important HSP genes in Arabidopsis. Expression of HSP genes BiP3, HSP70B, and HSP90.1 was positively regulated by GSH, and a promoter activation assay suggested a role for GSH in their induction. Lower expression of BiP3 and HSP70B in the GSH-fed Atmyb21 mutant and of HSP90.1 in the GSH-fed Atbzip10 mutant, in comparison with GSH-fed Col-0, revealed a role for GSH in activating their promoters through the transcription factors MYB21 and BZIP10. Co-transfection of transcription factor mutant protoplasts with transcription factor constructs and HSP promoters confirmed the results. Comparative proteomics also revealed proteins whose expression was controlled by MYB21 and BZIP10 in response to GSH feeding. A co-immunoprecipitation assay demonstrated a role for GSH in modulating the level of interaction of glutathione-S-transferase with HSP70. Collectively, our results demonstrate a role for GSH in activating the promoters of BiP3 and HSP70B via MYB21 and of HSP90.1 via BZIP10.
Pubmed ID: 29722824
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Commercial instrument and chemical vendor. Developer and manufacturer of specialized technological products for life science research and clinical diagnostics markets.
View all literature mentionsA collection of both commercial and noncommercial software products which includes: Mascot Distiller, Mascot Parser, and Mascot Server. Mascot Distiller is commercial and provides a single interface to process raw data into de-isotoped peak lists. This tool can also be used for the easy distribution of search and quantitative results to colleagues. The non-commercial Mascot Parser software provides an API (Application Programmer Interface) that makes it easier to access search results written in C++, Java, Python and Perl. Mascot Server is non-commercial, and is a collection of peptide mass fingerprints as well as a MS/MS database. A selection of popular sequence databases are available online and include SwissProt, NCBInr, and the EST divisions of EMBL. This server is best used for evaluating and searching for smaller data sets.
View all literature mentionsNational and international arabidopsis germplasm resource.Stores over million genotypes in physical stocks servicing worldwide Arabidopsis community in more than 50 countries. Stocks are provided under identical cost recovery conditions to academic and commercial researchers. Non-transgenic stocks are sent gratis to K-17 institutions and in special cases. Provides seed and information resources to International Arabidopsis Genome Programme and research community. Maintains accessions of Arabidopsis thaliana (and small number of other related species), including Characterized lines, Mapping populations, and Insertion lines. You may browse or search catalog.
View all literature mentionsDatabase of known and predicted protein interactions. The interactions include direct (physical) and indirect (functional) associations and are derived from four sources: Genomic Context, High-throughput experiments, (Conserved) Coexpression, and previous knowledge. STRING quantitatively integrates interaction data from these sources for a large number of organisms, and transfers information between these organisms where applicable. The database currently covers 5''214''234 proteins from 1133 organisms. (2013)
View all literature mentionsNASCArrays is the Nottingham Arabidopsis Stock Centre''s microarray database. Currently most of the data is for Arabidopsis thaliana experiments run by the NASC Affymetrix Facility. There are also experiments from other species, and experiments run by other centres too. NASCArrays is an Affymetrix microarray database. It contains free Affymetrix microarray data, and also features a series of tools allowing you to query that data in powerful ways. Most of the data currently comes from NASC''s Affymetrix Service. It also includes data from other sources, notably the AtGenExpress project. They currently distribute over 30,000 tubes of seed a year. There are currently the following data mining tools available. All of these tools allow you to type in a gene(s) of interest, and identify experiments or slides that you might be interested in: -Spot History: This tool allows you to see the pattern of gene expression over all slides in the database. Easily identify slides (and therefore experimental treatments) where genes are highly, lowly, or unusually expressed -Two gene scatter plot: This tool allows you to see the pattern of gene expression over all slides for two genes as a scatter plot. If you are interested in two genes, you can find out if they act in tandem, and highlight slides (and therefore experimental conditions) where these two genes behave in an unusual manner. -Gene Swinger: If you have a gene of interest, this tool will show you which experiment the gene expression varied most -Bulk Gene Download: This tool allows you to download the expression of a list of genes over all experiments. You can get all genes over all experiments (the entire database!) from the Super Bulk Gene Download Sponsors: This is a BBSRC funded consortium to provide services to the Arabidopsis community.
View all literature mentionsNational and international arabidopsis germplasm resource.Stores over million genotypes in physical stocks servicing worldwide Arabidopsis community in more than 50 countries. Stocks are provided under identical cost recovery conditions to academic and commercial researchers. Non-transgenic stocks are sent gratis to K-17 institutions and in special cases. Provides seed and information resources to International Arabidopsis Genome Programme and research community. Maintains accessions of Arabidopsis thaliana (and small number of other related species), including Characterized lines, Mapping populations, and Insertion lines. You may browse or search catalog.
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