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Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni.

Mingming Liu | Paul Franck Adjou Moumouni | Masahito Asada | Hassan Hakimi | Tatsunori Masatani | Patrick Vudriko | Seung-Hun Lee | Shin-Ichiro Kawazu | Junya Yamagishi | Xuenan Xuan
Parasites & vectors | 2018

Genetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis.

Pubmed ID: 29685172

Research resources used in this publication

None found

Antibodies used in this publication

None found

Associated grants

  • Agency: Japan Society for the Promotion of Science, International
    Id: Core-to-Core program
  • Agency: Ministry of Education, Culture, Sports, Science and Technology, International
    Id: 26304036

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GE Healthcare (tool)

RRID:SCR_000004

A commercial antibody supplier and provider of various services.

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GenBank (tool)

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NIH genetic sequence database that provides annotated collection of all publicly available DNA sequences for almost 280 000 formally described species (Jan 2014) .These sequences are obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects, including whole-genome shotgun (WGS) and environmental sampling projects. Most submissions are made using web-based BankIt or standalone Sequin programs, and GenBank staff assigns accession numbers upon data receipt. It is part of International Nucleotide Sequence Database Collaboration and daily data exchange with European Nucleotide Archive (ENA) and DNA Data Bank of Japan (DDBJ) ensures worldwide coverage. GenBank is accessible through NCBI Entrez retrieval system, which integrates data from major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of GenBank database are available by FTP.

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Thermo Fisher Scientific (tool)

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Commercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.

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QIAGEN (tool)

RRID:SCR_008539

A commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.

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