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A novel, fully-automated, chemiluminescent assay for the detection of 1,25-dihydroxyvitamin D in biological samples.

Andre Valcour | Claudia Zierold | Angela L Podgorski | Gregory T Olson | John V Wall | Hector F DeLuca | Fabrizio Bonelli
The Journal of steroid biochemistry and molecular biology | 2016

1,25-Dihydroxyvitamin D (1,25-(OH)2D), the hormonal form of vitamin D, is difficult to measure because of its low circulating levels (pg/mL), and similarity to more abundant metabolites. Here a fully-automated chemiluminescent assay that accurately and precisely measures 1,25-(OH)2D is described.

Pubmed ID: 26303747

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Microsoft Excel (tool)

RRID:SCR_016137

Software application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.

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