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Registered report: tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.

Denise Chroscinski | Darryl Sampey | Nimet Maherali | Reproducibility Project: Cancer Biology | Reproducibility Project Cancer Biology
eLife | 2015

The Nature in 2010 (Ricci-Vitiani et al., 2010). The experiments that will be replicated are those reported in Figure 4B and Supplementary Figure 10B (Ricci-Vitiani et al., 2010), which demonstrate that glioblastoma stem-like cells can derive into endothelial cells, and can be selectively ablated to reduce tumor progression in vivo, and Supplementary Figures S10C and S10D (Ricci-Vitiani et al., 2010), which demonstrate that fully differentiated glioblastoma cells cannot form functionally relevant endothelium. The Reproducibility Project: Cancer Biology is a collaboration between the eLife.

Pubmed ID: 25714925

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This is a list of tools and resources that we have found mentioned in this publication.


Center for Open Science (tool)

RRID:SCR_003239

Non-profit organization to increase the openness, integrity, and reproducibility of scientific research. COS priorities are to (1) make the entire scientific workflow accessible, (2) increase scientists' accountability, (3) facilitate good practices by scientists, (4) increase reward for good practices, and (5) promote reproducibility of scientific findings. COS has three primary activities: * Infrastructure development to support the scientific workflow and to connect technologies that support scientific research and communication, such as the Open Science Framework * Community building on open practices among open-source developers, open science researchers, and the broader scientific community, such as the Open Science Collaboration * Metascience, research on scientific practices, such as Reproducibility Project: Psychology and our partnership with Science Exchange, Reproducibility Project: Cancer Biology.

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ScienceExchange (tool)

RRID:SCR_010620

Access service resource which connects labs needing and offering experimental services. Users can search for academic and government labs and experimental services, request and compare service quotes, and directly communicate with labs to arrange orders.

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Novus Biologicals (tool)

RRID:SCR_004286

Commercial antibody vendor which supplies antibodies and other products to life science researchers.

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U-87MG ATCC (tool)

RRID:CVCL_0022

Cell line U-87MG ATCC is a Cancer cell line with a species of origin Homo sapiens (Human)

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U-87MG ATCC (tool)

RRID:CVCL_0022

Cell line U-87MG ATCC is a Cancer cell line with a species of origin Homo sapiens (Human)

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U-251MG (tool)

RRID:CVCL_0021

Cell line U-251MG is a Cancer cell line with a species of origin Homo sapiens (Human)

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HEK293T (tool)

RRID:CVCL_0063

Cell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)

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Crl:NU(NCr)-Foxn1nu (tool)

RRID:IMSR_CRL:490

Mus musculus with name Crl:NU(NCr)-Foxn1nu from IMSR.

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T98G (tool)

RRID:CVCL_0556

Cell line T98G is a Cancer cell line with a species of origin Homo sapiens (Human)

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HUVEC-C (tool)

RRID:CVCL_2959

Cell line HUVEC-C is a Finite cell line with a species of origin Homo sapiens

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GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

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Open Science Framework (storage service resource)

RRID:SCR_003238

Platform to support research and enable collaboration. Used to discover projects, data, materials, and collaborators helpful to your own research.

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G*Power (software resource)

RRID:SCR_013726

Data analytics software to compute statistical power analyses for many commonly used statistical tests in social and behavioral research. It can also be used to compute effect sizes and to graphically display the results of power analyses.

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R Project for Statistical Computing (software resource)

RRID:SCR_001905

Software environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.

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