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Widely used restriction-dependent cloning methods are labour-intensive and time-consuming, while several types of ligase-independent cloning approaches have inherent limitations. A rapid and reliable method of cloning native DNA sequences into desired plasmids are highly desired.
Pubmed ID: 22768962
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Cell line C2C12 is a Spontaneously immortalized cell line with a species of origin Mus musculus (Mouse)
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