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Most filarial parasite species contain Wolbachia, obligatory bacterial endosymbionts that are crucial for filarial development and reproduction. They are targets for alternative chemotherapy, but their role in the biology of filarial nematodes is not well understood. Light microscopy provides important information on morphology, localization and potential function of these bacteria. Surprisingly, immunohistology and in situ hybridization techniques have not been widely used to monitor Wolbachia distribution during the filarial life cycle.
Pubmed ID: 21629728
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Compact confocal microscope with highly sensitive GaAsP detection and fast linear scanning. The airyscan system allows capture images with high sensitivity and superresolution compared with conventional confocal microscope.
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