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Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1.

Jimmy H Saw | Bruce W Mountain | Lu Feng | Marina V Omelchenko | Shaobin Hou | Jennifer A Saito | Matthew B Stott | Dan Li | Guang Zhao | Junli Wu | Michael Y Galperin | Eugene V Koonin | Kira S Makarova | Yuri I Wolf | Daniel J Rigden | Peter F Dunfield | Lei Wang | Maqsudul Alam
Genome biology | 2008

Gram-positive bacteria of the genus Anoxybacillus have been found in diverse thermophilic habitats, such as geothermal hot springs and manure, and in processed foods such as gelatin and milk powder. Anoxybacillus flavithermus is a facultatively anaerobic bacterium found in super-saturated silica solutions and in opaline silica sinter. The ability of A. flavithermus to grow in super-saturated silica solutions makes it an ideal subject to study the processes of sinter formation, which might be similar to the biomineralization processes that occurred at the dawn of life.

Pubmed ID: 19014707

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Associated grants

  • Agency: Intramural NIH HHS, United States
    Id: Z99 LM999999

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CAP3 Sequence Assembly Program (tool)

RRID:SCR_007250

This form allows you to assemble a set of contiguous sequences (contigs) with the CAP3 program. The CAP3 program has a capability to clip 5'' and 3'' low-quality regions of reads. It uses base quality values in computation of overlaps between reads, construction of multiple sequence alignments of reads, and generation of consensus sequences. The program also uses forward-reverse constraints to correct assembly errors and link contigs. Results of CAP3 on four BAC data sets are presented. The performance of CAP3 was compared with that of PHRAP on a number of BAC data sets. PHRAP often produces longer contigs than CAP3 whereas CAP3 often produces fewer errors in consensus sequences than PHRAP. It is easier to construct scaffolds with CAP3 than with PHRAP on low-pass data with forward-reverse constraints. Sponsors: This project was supported by NIH Grant R01HG01502-02 from NHGRI. Keywords: CAP3, Program, Form, Computation, DNA, Dataset, Database, Program,

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